pUCPMA-M24 vector (V002354)

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LOCUS       40924_45388        4453 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Transient gene expression vector pUCPMA-M24, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Maiti IB, Dey N.
  TITLE     Further characterization and expression analysis of Mirabilis mosaic
            caulimovirus (MMV) full-length transcript promoter with single and 
            double enhancer domains in transgenic plants
  JOURNAL   Transgenics 3, 61-70 (1999)
REFERENCE   2  (bases 1 to 4453)
  AUTHORS   Dey N, Maiti IB.
  TITLE     Structure and promoter/leader deletion analysis of mirabilis mosaic 
            virus (MMV) full-length transcript promoter in transgenic plants
  JOURNAL   Plant Mol. Biol. 40 (5), 771-782 (1999)
  PUBMED    10487212
REFERENCE   3  (bases 1 to 4453)
  AUTHORS   Maiti IB, Dey N, Shepherd RJ.
  TITLE     Use of the full-length transcript (FLt) from Mirabilis mosaic 
            caulimovirus to express chimeric genes in plants
  JOURNAL   Patent: US US6420547B1-B 16-JUL-2002;
REFERENCE   4  (bases 1 to 4453)
  AUTHORS   Maiti IB, Dey N.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2015) KTRDC, Plant Genetic Engineering Research 
            and Services, College of Agriculture, University of Kentucky, 1401 
            University Drive, Lexington, KY 40546, USA
REFERENCE   5  (bases 1 to 4453)
  TITLE     Direct Submission
REFERENCE   6  (bases 1 to 4453)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Transgenics
            3, 61-70 (1999)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Plant Mol. 
            Biol."; date: "1999"; volume: "40"; issue: "5"; pages: "771-782"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Patent: US 
            US6420547B1-B 16-JUL-2002;"
COMMENT     SGRef: number: 4; type: "Journal Article"; journalName: "Submitted 
            (12-AUG-2015) KTRDC, Plant Genetic Engineering Research and 
            Services, College of Agriculture, University of Kentucky, 1401 
            University Drive, Lexington, KY 40546, USA"
COMMENT     SGRef: number: 5; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(6..647)
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     misc_feature    complement(647..692)
                     /note="multiple cloning site; XbaI-SacI-XhoI-BamHI-HindIII"
     regulatory      complement(692..1320)
                     /note="M24 promoter; derived from Mirabilis mosaic virus
                     (MMV)"
                     /regulatory_class="promoter"
     misc_feature    complement(1321..1326)
                     /label=EcoRI restriction enzyme site
                     /note="EcoRI restriction enzyme site"
     primer_bind     complement(1327..1343)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1556..2011
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2293..2397
                     /label=AmpR promoter
     CDS             2398..3255
                     /label=AmpR
                     /note="beta-lactamase"
     regulatory      3259..3417
                     /label=ampR terminator
                     /note="ampR terminator"
                     /regulatory_class="polyA_signal_sequence"
     rep_origin      3429..4017
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4305..4326
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4341..4371
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4379..4395
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4403..4419
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"