pUCP18T-miniTN7-lux-Gm vector (V002376)

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LOCUS       40924_45293       11888 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter vector pUCP18T-miniTN7-lux-Gm, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11888)
  AUTHORS   Damron FH, McKenney ES, Barbier M, Liechti GW, Schweizer HP, 
            Goldberg JB.
  TITLE     Construction of conjugatable Mini-Tn7 Vectors for Bioluminescent 
            Detection and Single Copy Promoter lux Reporter Analysis in 
            Gram-negative Bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11888)
  AUTHORS   Damron FH, McKenney ES, Barbier M, Liechti GW, Schweizer HP, 
            Goldberg JB.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAR-2013) Microbiology, Immunology, and Cancer 
            Biology, University of Virginia, PO Box 800734, Charlottesville, VA 
            22908, USA
REFERENCE   3  (bases 1 to 11888)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11888)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-MAR-2013) Microbiology, Immunology, and Cancer Biology, 
            University of Virginia, PO Box 800734, Charlottesville, VA 22908, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11888
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     349..365
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      393..487
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      590..676
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    722..749
                     /label=FRT
                     /note="FRT"
     CDS             complement(835..1365)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     regulatory      1424..1465
                     /label=promoter 2
                     /note="promoter 2"
                     /regulatory_class="promoter"
     promoter        complement(1554..1582)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    1623..1670
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        1766..1794
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     primer_bind     complement(2191..2213)
                     /label=Plux seq R
                     /note="Plux seq R"
     CDS             2641..4080
                     /label=LuxC
                     /note="LuxC fatty acid reductase"
     CDS             4095..5015
                     /label=LuxD
                     /note="LuxD acyltransferase"
     CDS             5067..6146
                     /label=LuxA
                     /note="LuxA luciferase subunit"
     CDS             6164..7144
                     /label=LuxB
                     /note="LuxB luciferase subunit"
     CDS             7326..8435
                     /label=LuxE
                     /note="LuxE"
     mobile_element  complement(9169..9334)
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     oriT            complement(9493..9601)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      complement(9833..10421)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10595..11452)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(11453..11557)
                     /label=AmpR promoter