pUChTNFR75f vector (V002378)

LOCUS       40924_45273        5095 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Bacterial expression vector pUChTNFR75f, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5095)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5095)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5095)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5095)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5095
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(403..1512)
                     /label=3UTR
                     /note="3UTR"
     CDS             complement(1513..2803)
                     /codon_start=1
                     /note="unnamed protein product; TNFRf"
                     /protein_id="SJL88754.1"
                     /translation="RPGAREHMPAQRIL*PDSSDVLQQMLAGPTCKSLLYQDLGHRV*L
                     L*GQHIHPALELGSRVLELWLPL*L*PGGNSSLHSGTEPHLHLQARLVLRAEQAGGVPA
                     VRAAAQVPPGLRRGQTRN*NIRRGVQALCPGDVLQHDFIHGYLQAPPDL*RGGHPWECK
                     HGCSLHVHVPHPEYGPRGSTLTPASVHTIPTHAANSRTQHCSKHLLPAPNGPQPPS*RE
                     HWRLRSSSWTDCGCDSLGSTNNRSGELCHHDPGEKEALVPAERSQGASLACR*GPGYTG
                     PRAAAPADHSAELQQQLPGELGQCVGQKGAHSEPATGTRRGGQWGRGGPGQHRELRFFP
                     WWPWDPGQCHLHRERL*QL*PQLTVLLPSQLHNGRHRFQPLGVPEGRAGPLLQGGMCLS
                     VTAGDARDPAGEHRREAPAPWSA*CWDEAQL"
     misc_feature    2805..2861
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2874..2890)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2898..2914)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2922..2952)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2967..2988)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3276..3864)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4038..4895)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4896..5000)
                     /label=AmpR promoter