pUCAG.nkx2.5 vector (V002385)

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LOCUS       V002385                 6175 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002385
VERSION     V002385
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6175)
  AUTHORS   van Tuyn J, Pijnappels DA, de Vries AA, de Vries I, van der
            Velde-van Dijke I, Knaan-Shanzer S, van der Laarse A, Schalij MJ,
            Atsma DE.
  TITLE     Fibroblasts from human postmyocardial infarction scars acquire
            properties of cardiomyocytes after transduction with a recombinant
            myocardin gene
  JOURNAL   FASEB J. 21 (12), 3369-3379 (2007)
   PUBMED   17579192
REFERENCE   2  (bases 1 to 6175)
  AUTHORS   van Tuyn J.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2006) MCB, LUMC, Einthovenweg 20, Leiden, ZH 2300
            RC, Netherlands
REFERENCE   3  (bases 1 to 6175)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6175)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FASEB J.";
            date: "2007"; volume: "21"; issue: "12"; pages: "3369-3379"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (13-DEC-2006) MCB, LUMC, Einthovenweg 20, Leiden, ZH 2300 RC,
            Netherlands"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6175
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      68..448
                     /label="CMV.IE enhancer"
                     /note="CMV.IE enhancer"
                     /regulatory_class="enhancer"
     enhancer        68..447
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        450..725
                     /label="chicken beta-actin promoter"
     intron          726..1734
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
     CDS             1845..2816
                     /gene="NKX2-5"
                     /label="Homeobox protein Nkx-2.5"
                     /note="Homeobox protein Nkx-2.5 from Homo sapiens.
                     Accession#: P52952"
     polyA_signal    3144..3199
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(3576..3592)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        4066..4170
                     /label="AmpR promoter"
     CDS             4171..5028
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5202..5790
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    6078..6099
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6114..6144
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    6152..6168
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."