pUC18_LexA+Cp190 vector (V002400)

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LOCUS       V002400                12082 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002400
VERSION     V002400
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 12082)
  AUTHORS   Posukh OV, Maksimov DA, Koryakov DE, Pokholkova GV, Skvortsova KN,
            Belyaeva ES, Belyakin SN.
  TITLE     Repressive Histone Marks Renewal Impedes Replication Fork
            Progression and Contributes To Late Replication in Drosophila
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12082)
  AUTHORS   Posukh OV, Maksimov DA, Koryakov DE, Pokholkova GV, Skvortsova KN,
            Belyaeva ES, Belyakin SN.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUN-2016) Genomics lab, IMCB SD RAS, Lavrentjeva ave.
            8/2, Novosibirsk, NSO 630090, Russia
REFERENCE   3  (bases 1 to 12082)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12082)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (03-JUN-2016) Genomics lab, IMCB SD RAS, Lavrentjeva ave. 8/2,
            Novosibirsk, NSO 630090, Russia"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12082
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    220..289
                     /label="attB"
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     protein_bind    534..567
                     /label="FRT (minimal)"
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     promoter        1448..1900
                     /label="hsp70 promoter"
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     complement(1970..1986)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             2004..2609
                     /label="LexA"
                     /note="E. coli DNA-binding protein that represses genes
                     involved in the SOS response to DNA damage"
     CDS             2619..2639
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             2652..5939
                     /gene="Cp190"
                     /label="Centrosome-associated zinc finger protein Cp190"
                     /note="Centrosome-associated zinc finger protein Cp190 from
                     Drosophila melanogaster. Accession#: Q24478"
     protein_bind    10161..10194
                     /label="FRT (minimal)"
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     rep_origin      complement(10263..10851)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(11025..11882)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(11883..11987)
                     /label="AmpR promoter"