pUC18VcluxOR vector (V002401)

LOCUS       40924_45123        4186 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pUC18VcluxOR DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Kasai S.
  TITLE     Freshwater bioluminescence in Vibrio albensis (Vibrio cholerae 
            biovar albensis) NCIMB 41 is caused by a two-nucleotide deletion in 
            luxO
  JOURNAL   J. Biochem. 139 (3), 471-482 (2006)
  PUBMED    16567412
REFERENCE   2  (bases 1 to 4186)
  AUTHORS   Kasai S.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2005) Contact:Sabu Kasai Graduate School of 
            Engineering, Osaka City University, Department of Applied Chemistry 
            and Bioapplied Chemistry; Sugimoto 3-3-138, Sumiyoshi-ku, Osaka 
            558-8585, Japan
REFERENCE   3  (bases 1 to 4186)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4186)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Biochem."; date: "2006"; volume: "139"; issue: "3"; pages: "471-482"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-SEP-2005) Contact:Sabu Kasai Graduate School of Engineering, 
            Osaka City University, Department of Applied Chemistry and 
            Bioapplied Chemistry; Sugimoto 3-3-138, Sumiyoshi-ku, Osaka 
            558-8585, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4186
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          2259..3783
                     /strain="NCIMB 41"
                     /mol_type="other DNA"
                     /note="biovar:albensis; synonym: Vibrio cholerae bv.
                     albensis"
                     /db_xref="taxon:140100"
                     /organism="Vibrio albensis"
     promoter        96..200
                     /label=AmpR promoter
     CDS             201..1058
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1232..1820
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2144..2174
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2412..3779
                     /codon_start=1
                     /gene="luxO"
                     /product="LuxO"
                     /label=luxO
                     /note="derived from Vibrio cholerae biovar albensis lux0
                     gene, deposited in Acc# AB114423, a two nucleotide deletion
                     is recoverd"
                     /protein_id="BAE87124.1"
                     /translation="MVEDTASVAALYRSYLTPLDIDINIVGTGRDAIESIGRREPDLIL
                     LDLRLPDMTGMDVLYAVKEKSPDVPIVFMTAHGSIDTAVEAMRHGTQDFLIKPCEADRL
                     RVTVNNAIRKASKLKNDVDNKNQNYQGFIGSSQTMQAVSRTIDSAASSKASIFITGESG
                     TGKEVCAEAIHAASKRGDKPFIAINCAAIPKDLIESELFGHVKGAFTGAATERQGAAEA
                     ADGGTLFLDELCEMDLDLQTKLLRFIQTGTFQKVGSSKMKSVDVRFVCATNRDPWKEVQ
                     EGRFREDLYYRLYVIPLHLPPLRARGDDVIEIAYSLLGFMSKEEGKDFVRLSAEVVERF
                     RQYEWPGNVRQLQNVLRNVVVLNEGREITLDMLPPPLNQMSVPINRALPLAHENKVSVH
                     EIFPLWMTEKQAIEQAIEACDGNIPRAATYLDVSPSTIYRKLQTWNEKVKEKEKER"
     gene            2412..3779
                     /gene="luxO"
                     /label=luxO
     primer_bind     complement(3792..3808)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"