pUbiGUSPlus vector (V002448)

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LOCUS       40924_44854        7018 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter vector pUbiGUSPlus, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7018)
  AUTHORS   Vickers CE, Xue GP, Gresshoff PM.
  TITLE     A synthetic xylanase as a novel reporter in plants
  JOURNAL   Plant Cell Rep. 22 (2), 135-140 (2003)
  PUBMED    12845475
REFERENCE   2  (bases 1 to 7018)
  AUTHORS   Vickers CE.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-OCT-2003) ARC Centre for Integrative Legume Research, 
            The University of Queensland, Room 213, John Hines Building (69), 
            St. Lucia, QLD 4072, Australia
REFERENCE   3  (bases 1 to 7018)
  AUTHORS   Vickers CE.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-FEB-2004) ARC Centre for Integrative Legume Research, 
            The University of Queensland, Room 213, John Hines Building (69), 
            St. Lucia, QLD 4072, Australia
REFERENCE   4  (bases 1 to 7018)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 7018)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Rep."; date: "2003"; volume: "22"; issue: "2"; pages: "135-140"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-OCT-2003) ARC Centre for Integrative Legume Research, The 
            University of Queensland, Room 213, John Hines Building (69), St. 
            Lucia, QLD 4072, Australia"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (20-FEB-2004) ARC Centre for Integrative Legume Research, The 
            University of Queensland, Room 213, John Hines Building (69), St. 
            Lucia, QLD 4072, Australia"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     On Feb 20, 2004 this sequence version replaced AY452736.1.
FEATURES             Location/Qualifiers
     source          1..7018
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     319..335
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        351..2342
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     misc_feature    2356..2388
                     /note="synthetic untranslated leader sequence; sUTR"
     intron          2404..2593
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             2588..4411
                     /label=GUSPlus(TM)
                     /note="beta-glucuronidase"
     CDS             4418..4435
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      4467..4719
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4737..4753)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4761..4777)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4785..4815)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4830..4851)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5139..5727)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5901..6758)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6759..6863)
                     /label=AmpR promoter