Basic Vector Information
- Vector Name:
- pUB-GWS-Hyg
- Antibiotic Resistance:
- Kanamycin
- Length:
- 14183 bp
- Type:
- Binary vector
- Replication origin:
- ori
- Host:
- Plants
- Source/Author:
- Maekawa T, Kusakabe M, Shimoda Y, Sato S, Tabata S, Murooka Y, Hayashi M.
- Promoter:
- CaMV 35S (enhanced)
pUB-GWS-Hyg vector Vector Map
pUB-GWS-Hyg vector Sequence
LOCUS 40924_44824 14183 bp DNA circular SYN 18-DEC-2018
DEFINITION Binary vector pUB-GWS-Hyg DNA, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 14183)
AUTHORS Maekawa T, Kusakabe M, Shimoda Y, Sato S, Tabata S, Murooka Y,
Hayashi M.
TITLE Polyubiquitin Promoter-Based Binary Vectors for Overexpression and
Gene Silencing in Lotus japonicus
JOURNAL Mol. Plant Microbe Interact. 21 (4), 375-382 (2008)
PUBMED 18321183
REFERENCE 2 (bases 1 to 14183)
AUTHORS Maekawa T, Hayashi M.
TITLE Direct Submission
JOURNAL Submitted (09-MAY-2007) Contact:Makoto Hayashi National Institute of
Agrobiological Sciences, Environmental Stress Research Unit; 2-1-2
Kannondai, Tsukuba, Ibaraki 305-8602, Japan
REFERENCE 3 (bases 1 to 14183)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 14183)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Plant
Microbe Interact."; date: "2008"; volume: "21"; issue: "4"; pages:
"375-382"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(09-MAY-2007) Contact:Makoto Hayashi National Institute of
Agrobiological Sciences, Environmental Stress Research Unit; 2-1-2
Kannondai, Tsukuba, Ibaraki 305-8602, Japan"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..14183
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 1..25
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
polyA_signal complement(103..277)
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
CDS complement(320..1342)
/label=HygR
/note="aminoglycoside phosphotransferase from E. coli"
promoter complement(1410..2087)
/label=CaMV 35S promoter (enhanced)
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
protein_bind 2278..2299
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2314..2344
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2352..2368
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2376..2392
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
terminator complement(2414..2666)
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
protein_bind 2743..2867
/label=attR1
/note="recombination site for the Gateway(R) LR reaction"
promoter 2892..2922
/label=lac UV5 promoter
/note="E. coli lac promoter with an 'up' mutation"
CDS 2976..3632
/label=CmR
/note="chloramphenicol acetyltransferase"
CDS 3977..4279
/label=ccdB
/note="CcdB, a bacterial toxin that poisons DNA gyrase"
protein_bind complement(4323..4447)
/label=attR2
/note="recombination site for the Gateway(R) LR reaction"
misc_feature 4481..4735
/note="contains intron 1 of Arabidopsis thaliana WRKY
transcription factor 33"
protein_bind 4790..4914
/label=attR2
/note="recombination site for the Gateway(R) LR reaction"
CDS complement(4958..5260)
/label=ccdB
/note="CcdB, a bacterial toxin that poisons DNA gyrase"
CDS complement(5605..6261)
/label=CmR
/note="chloramphenicol acetyltransferase"
promoter complement(6315..6345)
/label=lac UV5 promoter
/note="E. coli lac promoter with an 'up' mutation"
protein_bind complement(6370..6494)
/label=attR1
/note="recombination site for the Gateway(R) LR reaction"
primer_bind complement(6522..6538)
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
gene complement(6539..7125)
/gene="Ljubq1"
/label=Ljubq1
intron complement(6539..7078)
/gene="Ljubq1"
/note="5' UTR intron of Ljubq1 gene"
exon complement(7079..7125)
/gene="Ljubq1"
/number=1
regulatory complement(7126..7660)
/label=Ljubq1 promoter
/note="Ljubq1 promoter"
/regulatory_class="promoter"
primer_bind complement(7687..7703)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 7906..7930
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
CDS 9230..9856
/label=pVS1 StaA
/note="stability protein from the Pseudomonas plasmid pVS1
(Heeb et al., 2000)"
CDS 10293..11357
/label=pVS1 RepA
/note="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
rep_origin 11426..11620
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
misc_feature 11964..12104
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(12290..12878)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(12968..13759)
/label=KanR
/note="aminoglycoside phosphotransferase"
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