Basic Vector Information
- Vector Name:
- pUB-Cas9-@GL1
- Antibiotic Resistance:
- Kanamycin
- Length:
- 14834 bp
- Type:
- Binary vector
- Replication origin:
- ori
- Host:
- Plants
- Source/Author:
- Hahn F, Mantegazza O, Greiner A, Hegemann P, Eisenhut M, Weber AP.
- Promoter:
- CaMV 35S (enhanced)
pUB-Cas9-@GL1 vector Vector Map
pUB-Cas9-@GL1 vector Sequence
LOCUS 40924_44789 14834 bp DNA circular SYN 18-DEC-2018
DEFINITION Binary vector pUB-Cas9-@GL1, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 14834)
AUTHORS Hahn F, Mantegazza O, Greiner A, Hegemann P, Eisenhut M, Weber AP.
TITLE An Efficient Visual Screen for CRISPR/Cas9 Activity in Arabidopsis
thaliana
JOURNAL Front Plant Sci 8, 39 (2017)
PUBMED 28174584
REFERENCE 2 (bases 1 to 14834)
AUTHORS Hahn F, Mantegazza O, Eisenhut M, Greiner A, Hegemann P, Weber APM.
TITLE Direct Submission
JOURNAL Submitted (03-NOV-2016) Plant Biochemistry, Heinrich Heine
University Dusseldorf, Universitatsstr. 1, Dusseldorf 40225, Germany
REFERENCE 3 (bases 1 to 14834)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 14834)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
Sci 8, 39 (2017)"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(03-NOV-2016) Plant Biochemistry, Heinrich Heine University
Dusseldorf, Universitatsstr. 1, Dusseldorf 40225, Germany"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..14834
/mol_type="other DNA"
/organism="synthetic DNA construct"
polyA_signal 40..214
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
misc_feature complement(292..316)
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
CDS 741..1532
/label=KanR
/note="aminoglycoside phosphotransferase"
rep_origin 1622..2210
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature complement(2396..2536)
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(2880..3074)
/direction=LEFT
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
CDS complement(3143..4207)
/label=pVS1 RepA
/note="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
CDS complement(4644..5270)
/label=pVS1 StaA
/note="stability protein from the Pseudomonas plasmid pVS1
(Heeb et al., 2000)"
misc_feature complement(6570..6594)
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
primer_bind 6797..6813
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
regulatory 6846..6855
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
regulatory 6850..6859
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
promoter 6903..7326
/label=AtU6-26
/note="Arabidopsis U6-26 gene promoter"
misc_feature 7336..7355
/label=GL1 protospacer sequence
/note="GL1 protospacer sequence"
misc_RNA 7356..7431
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
regulatory 7556..8195
/label=Arabidopsis thaliana Ubiquitin10 promoter
/note="Arabidopsis thaliana Ubiquitin10 promoter"
/regulatory_class="promoter"
protein_bind 8235..8259
/label=attB1
/note="recombination site for the Gateway(R) BP reaction"
CDS 8280..8300
/label=SV40 NLS
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
CDS 8298..12401
/label=Cas9
/note="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
protein_bind complement(12406..12430)
/label=attB2
/note="recombination site for the Gateway(R) BP reaction"
terminator 12496..12743
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
primer_bind complement(12759..12775)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 12783..12799
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(12807..12837)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(12852..12873)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 13064..13741
/label=CaMV 35S promoter (enhanced)
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
CDS 13809..14831
/label=HygR
/note="aminoglycoside phosphotransferase from E. coli"
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