pUASTattB-LT3-Dam vector (V002462)

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LOCUS       V002462                10106 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002462
VERSION     V002462
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10106)
  AUTHORS   Southall TD, Gold KS, Egger B, Davidson CM, Caygill EE, Marshall OJ,
            Brand AH.
  TITLE     Cell-type-specific profiling of gene expression and chromatin
            binding without cell isolation: assaying RNA Pol II occupancy in
            neural stem cells
  JOURNAL   Dev. Cell 26 (1), 101-112 (2013)
   PUBMED   23792147
REFERENCE   2  (bases 1 to 10106)
  AUTHORS   Southall TD, Brand AH.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2016) Life Sciences, Imperial College London, Sir
            Ernst Chain Building, London SW7 2AZ, United Kingdom
REFERENCE   3  (bases 1 to 10106)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10106)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Dev. Cell";
            date: "2013"; volume: "26"; issue: "1"; pages: "101-112"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-FEB-2016) Life Sciences, Imperial College London, Sir Ernst
            Chain Building, London SW7 2AZ, United Kingdom"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10106
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..459
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        623..641
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            666..4802
                     /label="mini-white"
                     /note="This modified version of the white gene lacks part
                     of the first intron."
     protein_bind    4809..4842
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4875..4969
                     /label="5X UAS"
                     /note="five tandem copies of the 'ScaI site' 17-mer
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS)
                     that efficiently binds yeast Gal4 (Webster et al., 1988;
                     Pfeiffer et al., 2010)"
     promoter        4988..5226
                     /label="hsp70 promoter"
                     /note="Drosophila melanogaster hsp70Bb promoter"
     CDS             5261..5968
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     CDS             5991..6824
                     /gene="dam"
                     /label="DNA adenine methylase"
                     /note="DNA adenine methylase from Escherichia coli (strain
                     K12). Accession#: P0AEE8"
     CDS             6831..6860
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     intron          6980..7045
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7175..7195
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    7467..7601
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     protein_bind    7630..7699
                     /label="attB"
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     promoter        complement(7918..7936)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7957..7973)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    7981..7997
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8005..8035)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8050..8071)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8359..8947)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9121..9978)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9979..10083)
                     /label="AmpR promoter"