pUAST-NTAP(GS) vector (V002465)

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LOCUS       40924_44749        9626 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUAST-NTAP(GS), complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9626)
  AUTHORS   Kyriakakis P, Tipping M, Abed L, Veraksa A.
  TITLE     Tandem affinity purification in Drosophila: The advantages of the 
            GS-TAP system
  JOURNAL   Fly (Austin) 2 (4) (2008) In press
  PUBMED    18719405
REFERENCE   2  (bases 1 to 9626)
  AUTHORS   Veraksa A, Kyriakakis P, Tipping M, Abed L.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2008) Biology Department, University of 
            Massachusetts Boston, 100 Morrissey Blvd., Boston, MA 02125, USA
REFERENCE   3  (bases 1 to 9626)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9626)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fly 
            (Austin) 2 (4) (2008) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-APR-2008) Biology Department, University of Massachusetts 
            Boston, 100 Morrissey Blvd., Boston, MA 02125, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9626
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(997..1854)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(1855..1959)
                     /label=AmpR promoter
     misc_feature    complement(2761..2993)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    3055..3149
                     /label=5X UAS
                     /note="five tandem copies of the 'ScaI site' 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        3168..3406
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     CDS             3809..3829
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     CDS             3836..3856
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     CDS             3863..3976
                     /label=SBP
                     /note="streptavidin-binding peptide"
     misc_feature    3979..4024
                     /note="polylinker; unique cloning sites for EcoRI,
                     EagI/NotI, XhoI, KpnI, XbaI"
     intron          4102..4167
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4297..4317
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    4742..4876
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     gene            4894..9030
                     /label=mini-white
                     /note="This modified version of the white gene lacks part
                     of the first intron."
     misc_feature    complement(9041..9626)
                     /label=P element 5' end