Basic Vector Information
- Vector Name:
- pUAST-CTAP(SG)
- Antibiotic Resistance:
- Ampicillin
- Length:
- 9621 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Kyriakakis P, Tipping M, Abed L, Veraksa A.
- Promoter:
- hsp70
pUAST-CTAP(SG) vector Map
pUAST-CTAP(SG) vector Sequence
LOCUS 40924_44739 9621 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pUAST-CTAP(SG), complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9621) AUTHORS Kyriakakis P, Tipping M, Abed L, Veraksa A. TITLE Tandem affinity purification in Drosophila: The advantages of the GS-TAP system JOURNAL Fly (Austin) 2 (4) (2008) In press PUBMED 18719405 REFERENCE 2 (bases 1 to 9621) AUTHORS Veraksa A, Kyriakakis P, Tipping M, Abed L. TITLE Direct Submission JOURNAL Submitted (20-APR-2008) Biology Department, University of Massachusetts Boston, 100 Morrissey Blvd., Boston, MA 02125, USA REFERENCE 3 (bases 1 to 9621) TITLE Direct Submission REFERENCE 4 (bases 1 to 9621) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Fly (Austin) 2 (4) (2008) In press" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (20-APR-2008) Biology Department, University of Massachusetts Boston, 100 Morrissey Blvd., Boston, MA 02125, USA" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..9621 /mol_type="other DNA" /organism="synthetic DNA construct" rep_origin complement(235..823) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(997..1854) /label=AmpR /note="beta-lactamase" promoter complement(1855..1959) /label=AmpR promoter misc_feature complement(2761..2993) /label=P element 3' end /note="P element 3' end" protein_bind 3055..3149 /label=5X UAS /note="five tandem copies of the 'ScaI site' 17-mer CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) that efficiently binds yeast Gal4 (Webster et al., 1988; Pfeiffer et al., 2010)" promoter 3168..3406 /label=hsp70 promoter /note="Drosophila melanogaster hsp70Bb promoter" misc_feature 3420..3465 /note="polylinker; unique cloning sites for EcoRI, NotI (EagI cannot be used), XhoI, KpnI, XbaI" CDS 3469..3582 /label=SBP /note="streptavidin-binding peptide" CDS 3589..3609 /label=TEV site /note="tobacco etch virus (TEV) protease recognition and cleavage site" CDS 3616..3636 /label=TEV site /note="tobacco etch virus (TEV) protease recognition and cleavage site" intron 4097..4162 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 4292..4312 /label=SV40 NLS /note="nuclear localization signal of SV40 (simian virus 40) large T antigen" polyA_signal 4737..4871 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" gene 4889..9025 /label=mini-white /note="This modified version of the white gene lacks part of the first intron." misc_feature complement(9036..9621) /label=P element 5' end
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