pUAST-C5 vector (V002468)

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LOCUS       40924_44734        9110 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning/transgenesis vector pUAST-C5, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9110)
  AUTHORS   Le T, Yu M, Williams B, Goel S, Paul SM, Beitel GJ.
  TITLE     CaSpeR5, a family of Drosophila transgenesis and shuttle vectors 
            with improved multiple cloning sites
  JOURNAL   BioTechniques 42 (2), 164 (2007)
  PUBMED    17373479
REFERENCE   2  (bases 1 to 9110)
  AUTHORS   Beitel GJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2006) BMBCB, Northwestern University, 2205 Tech 
            Dr., Evanston, IL 60091, USA
REFERENCE   3  (bases 1 to 9110)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9110)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "BioTechniques"; date: "2007"; volume: "42"; issue: "2"; pages: 
            "164"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-OCT-2006) BMBCB, Northwestern University, 2205 Tech Dr., 
            Evanston, IL 60091, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9110
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(997..1854)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(1855..1959)
                     /label=AmpR promoter
     misc_feature    complement(2761..2993)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    3055..3149
                     /label=5X UAS
                     /note="five tandem copies of the 'ScaI site' 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        3168..3406
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     misc_feature    3426..3649
                     /note="ppCasper5 multiple cloning site but with mutated
                     SphI site to eliminate ATG in the multiple cloning site"
     intron          3738..3803
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             3933..3953
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    4225..4359
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     gene            4377..8514
                     /label=mini-white
                     /note="This modified version of the white gene lacks part
                     of the first intron."
     misc_feature    complement(8525..9110)
                     /label=P element 5' end