pUAST-attB-stop-SuVar-MycDam vector (V002470)

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LOCUS       V002470                14300 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002470
VERSION     V002470
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 14300)
  AUTHORS   Posukh OV, Maksimov DA, Koryakov DE, Pokholkova GV, Skvortsova KN,
            Belyaeva ES, Belyakin SN.
  TITLE     Repressive histone marks renewal impedes replication fork
            progression and contributes to late replication in Drosophila
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14300)
  AUTHORS   Belyakin SN.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2015) Genomics Lab, IMCB SD RAS, Lavrentjeva Ave.
            8/2, Novosibirsk, NSO 630090, Russia
REFERENCE   3  (bases 1 to 14300)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 14300)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-OCT-2015) Genomics Lab, IMCB SD RAS, Lavrentjeva Ave. 8/2,
            Novosibirsk, NSO 630090, Russia"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14300
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    62..156
                     /label="5X UAS"
                     /note="five tandem copies of the 'ScaI site' 17-mer
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS)
                     that efficiently binds yeast Gal4 (Webster et al., 1988;
                     Pfeiffer et al., 2010)"
     promoter        175..413
                     /label="hsp70 promoter"
                     /note="Drosophila melanogaster hsp70Bb promoter"
     protein_bind    469..502
                     /label="loxP"
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
     polyA_signal    529..1401
                     /label="hsp70 poly(A)"
                     /note="polyadenlyation signal from the Drosophila
                     melanogaster hsp70Ab gene"
     protein_bind    complement(2585..2618)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             2718..4622
                     /gene="Su(var)3-9"
                     /label="Histone-lysine N-methyltransferase Su(var)3-9"
                     /note="Histone-lysine N-methyltransferase Su(var)3-9 from
                     Drosophila melanogaster. Accession#: P45975"
     CDS             4638..4667
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label="Myc"
                     /translation="EQKLISEEDL"
     CDS             4677..5507
                     /gene="dam"
                     /label="DNA adenine methylase"
                     /note="DNA adenine methylase from Escherichia coli (strain
                     K12). Accession#: P0AEE8"
     intron          5602..5667
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5797..5817
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    6089..6223
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     protein_bind    10299..10368
                     /label="attB"
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     misc_feature    complement(10722..11307)
                     /label="P element 5' end"
     rep_origin      complement(11542..12130)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(12304..13161)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(13162..13266)
                     /label="AmpR promoter"
     misc_feature    complement(14068..14300)
                     /label="P element 3' end"
                     /note="P element 3' end"