pUAST-attB-hsp70-stop-dam-comr vector (V002472)

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LOCUS       V002472                14180 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002472
VERSION     V002472
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 14180)
  AUTHORS   Laktionov P, Maksimov D, White-Cooper H, Belyakin S.
  TITLE     Cookie monster binding to the repressed chromosomal domains is
            needed for selective activation of testis-specific genes in
            Drosophila melanogaster
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14180)
  AUTHORS   Laktionov PP, Belyakin SN.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2013) Genomics lab, Institute of molecular and
            cellular biology, Lavrentiev ave 8/2, Novosibirsk 630090, Russia
REFERENCE   3  (bases 1 to 14180)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 14180)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (29-MAR-2013) Genomics lab, Institute of molecular and cellular
            biology, Lavrentiev ave 8/2, Novosibirsk 630090, Russia"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14180
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    48..142
                     /label="5X UAS"
                     /note="five tandem copies of the 'ScaI site' 17-mer
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS)
                     that efficiently binds yeast Gal4 (Webster et al., 1988;
                     Pfeiffer et al., 2010)"
     promoter        161..399
                     /label="hsp70 promoter"
                     /note="Drosophila melanogaster hsp70Bb promoter"
     misc_recomb     455..488
                     /label="loxP recombination site"
                     /note="loxP recombination site"
     protein_bind    455..488
                     /label="loxP"
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
     polyA_signal    515..1387
                     /label="hsp70 poly(A)"
                     /note="polyadenlyation signal from the Drosophila
                     melanogaster hsp70Ab gene"
     protein_bind    complement(2572..2605)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             2657..3490
                     /gene="dam"
                     /label="DNA adenine methylase"
                     /note="DNA adenine methylase from Escherichia coli (strain
                     K12). Accession#: P0AEE8"
     CDS             3497..3526
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     intron          5482..5547
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5677..5697
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    5969..6103
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     gene            6455..10000
                     /gene="mini-white"
                     /label="mini-white"
     protein_bind    10179..10248
                     /label="attB"
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     misc_feature    complement(10602..11187)
                     /label="P element 5' end"
     rep_origin      complement(11422..12010)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(12184..13041)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(13042..13146)
                     /label="AmpR promoter"
     misc_feature    complement(13948..14180)
                     /label="P element 3' end"
                     /note="P element 3' end"