pUAST-attB-Dam plasmid pUAST vector (V002473)

LOCUS       V002473                12343 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002473
VERSION     V002473
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 12343)
  AUTHORS   Belyakin S, Maksimov D.
  TITLE     SUUR binding to chromosomes is developmentally regulated and affects
            tissue-specific genes in Drosophila melanogaster
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12343)
  AUTHORS   Belyakin S, Maksimov D.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2011) MCB, ICBFM, Lavrentjeva Ave. 8, Novosibirsk,
            NSO 630090, Russia
REFERENCE   3  (bases 1 to 12343)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12343)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-NOV-2011) MCB, ICBFM, Lavrentjeva Ave. 8, Novosibirsk, NSO
            630090, Russia"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12343
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    48..142
                     /label="5X UAS"
                     /note="five tandem copies of the 'ScaI site' 17-mer
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS)
                     that efficiently binds yeast Gal4 (Webster et al., 1988;
                     Pfeiffer et al., 2010)"
     promoter        161..399
                     /label="hsp70 promoter"
                     /note="Drosophila melanogaster hsp70Bb promoter"
     protein_bind    complement(455..488)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    515..1387
                     /label="hsp70 poly(A)"
                     /note="polyadenlyation signal from the Drosophila
                     melanogaster hsp70Ab gene"
     protein_bind    complement(2572..2605)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             2657..3490
                     /gene="dam"
                     /label="DNA adenine methylase"
                     /note="DNA adenine methylase from Escherichia coli (strain
                     K12). Accession#: P0AEE8"
     CDS             3497..3526
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     intron          3645..3710
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             3840..3860
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    4132..4266
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     protein_bind    8342..8411
                     /label="attB"
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     misc_feature    complement(8765..9350)
                     /label="P element 5' end"
     rep_origin      complement(9585..10173)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(10347..11204)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(11205..11309)
                     /label="AmpR promoter"
     misc_feature    complement(12111..12343)
                     /label="P element 3' end"
                     /note="P element 3' end"