Basic Vector Information
- Vector Name:
- pUASg.attB
- Antibiotic Resistance:
- Ampicillin
- Length:
- 10293 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Bischof J, Bjorklund M, Furger E, Schertel C, Taipale J, Basler K.
- Promoter:
- hsp70
pUASg.attB vector Map
pUASg.attB vector Sequence
LOCUS 40924_44699 10293 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pUASg.attB, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10293) AUTHORS Bischof J, Bjorklund M, Furger E, Schertel C, Taipale J, Basler K. TITLE A versatile platform for creating a comprehensive UAS-ORFeome library in Drosophila JOURNAL Development (2013) In press PUBMED 23637332 REFERENCE 2 (bases 1 to 10293) AUTHORS Bischof J, Bjorklund M, Furger E, Schertel C, Taipale J, Basler K. TITLE Direct Submission JOURNAL Submitted (13-APR-2013) Institute of Molecular Life Sciences, University of Zurich, Winterthurerstrasse 190, Zurich 8057, Switzerland REFERENCE 3 (bases 1 to 10293) TITLE Direct Submission REFERENCE 4 (bases 1 to 10293) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Development (2013) In press" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (13-APR-2013) Institute of Molecular Life Sciences, University of Zurich, Winterthurerstrasse 190, Zurich 8057, Switzerland" COMMENT SGRef: number: 3; type: "Journal Article" COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..10293 /mol_type="other DNA" /organism="synthetic DNA construct" rep_origin complement(3..458) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind 600..616 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 623..641 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" gene 666..4802 /label=mini-white /note="This modified version of the white gene lacks part of the first intron." protein_bind 4842..4936 /label=5X UAS /note="five tandem copies of the 'ScaI site' 17-mer CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) that efficiently binds yeast Gal4 (Webster et al., 1988; Pfeiffer et al., 2010)" promoter 4955..5193 /label=hsp70 promoter /note="Drosophila melanogaster hsp70Bb promoter" protein_bind 5236..5360 /label=attR1 /note="recombination site for the Gateway(R) LR reaction" promoter 5385..5415 /label=lac UV5 promoter /note="E. coli lac promoter with an 'up' mutation" CDS 5469..6146 /label=CmR /note="chloramphenicol acetyltransferase" CDS 6469..6771 /label=ccdB /note="CcdB, a bacterial toxin that poisons DNA gyrase" protein_bind complement(6815..6939) /label=attR2 /note="recombination site for the Gateway(R) LR reaction" regulatory 6953..7765 /label=tubulin alpha 1 terminator /note="tubulin alpha 1 terminator" /regulatory_class="terminator" protein_bind 7817..7886 /label=attB /note="attB site for the phi-C31 integrase (Groth et al., 2000)" promoter complement(8105..8123) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(8144..8160) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind 8168..8184 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8192..8222) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(8237..8258) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(8546..9134) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(9308..10165) /label=AmpR /note="beta-lactamase" promoter complement(10166..10270) /label=AmpR promoter
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