Basic Vector Information
- Vector Name:
- pTY13-HisTAL
- Antibiotic Resistance:
- Kanamycin
- Length:
- 9948 bp
- Type:
- Cloning vector
- Replication origin:
- p15A ori
- Source/Author:
- Kim B, Binkley R, Kim HU, Lee SY.
- Promoter:
- trc
pTY13-HisTAL vector Vector Map
pTY13-HisTAL vector Sequence
LOCUS V002490 9948 bp DNA circular SYN 18-DEC-2018
DEFINITION Exported.
ACCESSION V002490
VERSION V002490
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 9948)
AUTHORS Kim B, Binkley R, Kim HU, Lee SY.
TITLE Metabolic engineering of Escherichia coli for the enhanced
production of l-tyrosine
JOURNAL Biotechnol. Bioeng. (2018) In press
PUBMED 30019750
REFERENCE 2 (bases 1 to 9948)
AUTHORS Yang D, Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY.
TITLE Direct Submission
JOURNAL Submitted (15-JUN-2018) Dept. Chemical and Biomolecular Engineering,
Korea Advanced Institute of Science and Technology, Daehak-ro 291,
Daejeon 34141, South Korea
REFERENCE 3 (bases 1 to 9948)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 9948)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
Bioeng. (2018) In press"
SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(15-JUN-2018) Dept. Chemical and Biomolecular Engineering, Korea
Advanced Institute of Science and Technology, Daehak-ro 291, Daejeon
34141, South Korea"
SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..9948
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 70..99
/label="trc promoter"
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
protein_bind 107..123
/label="lac operator"
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
CDS 143..1021
/gene="tyrC"
/label="Cyclohexadienyl dehydrogenase"
/note="Cyclohexadienyl dehydrogenase from Zymomonas mobilis
subsp. mobilis (strain ATCC 31821 / ZM4 / CP4). Accession#:
Q04983"
terminator 1277..1363
/label="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 1455..1482
/label="rrnB T2 terminator"
/note="transcription terminator T2 from the E. coli rrnB
gene"
promoter 1547..1575
/label="tac promoter"
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
protein_bind 1583..1599
/label="lac operator"
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
CDS 1622..2671
/gene="aroG"
/label="Phospho-2-dehydro-3-deoxyheptonate aldolase,
Phe-sensitive"
/note="Phospho-2-dehydro-3-deoxyheptonate aldolase,
Phe-sensitive from Escherichia coli (strain K12).
Accession#: P0AB91"
CDS 2712..3233
/gene="aroL"
/label="Shikimate kinase 2"
/note="Shikimate kinase 2 from Escherichia coli (strain
55989 / EAEC). Accession#: B7L543"
regulatory 3337..3662
/label="rrnBT1T2"
/note="rrnBT1T2"
/regulatory_class="terminator"
terminator 3457..3543
/gene="Escherichia coli rrnB"
/label="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 3635..3662
/label="rrnB T2 terminator"
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator 3975..4061
/gene="Escherichia coli rrnB"
/label="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 4153..4180
/label="rrnB T2 terminator"
/note="transcription terminator T2 from the E. coli rrnB
gene"
promoter 4200..4291
/label="AmpR promoter"
primer_bind complement(4604..4620)
/label="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
CDS 4863..5675
/label="KanR"
/note="aminoglycoside phosphotransferase"
rep_origin 6437..6982
/label="p15A ori"
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin."
regulatory 7417..7470
/label="Trc promoter"
/note="Trc promoter"
/regulatory_class="promoter"
promoter 7417..7446
/label="trc promoter"
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
protein_bind 7454..7470
/label="lac operator"
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
CDS 7499..7516
/label="6xHis"
/note="6xHis affinity tag"
terminator complement(9296..9339)
/label="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 9471..9498
/label="rrnB T2 terminator"
/note="transcription terminator T2 from the E. coli rrnB
gene"
regulatory 9882..9910
/label="Tac promoter"
/note="Tac promoter"
/regulatory_class="promoter"
promoter 9882..9910
/label="tac promoter"
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
protein_bind 9918..9934
/label="lac operator"
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
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