pTtag vector (V002498)

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LOCUS       40924_44404        6355 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Transformation vector pTtag, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6355)
  AUTHORS   El-Haddad H, Przyborski JM, Kraft LG, McFadden GI, Waller RF, Gould 
            SB.
  TITLE     Characterization of TtALV2, an Essential Charged Repeat Motif 
            Protein of the Tetrahymena thermophila Membrane Skeleton
  JOURNAL   Eukaryotic Cell 12 (6), 932-940 (2013)
  PUBMED    23606287
REFERENCE   2  (bases 1 to 6355)
  AUTHORS   Gould SB, McFadden GI, Waller RF.
  TITLE     Analysis of the two alveolate-characterizing epiplasmin proteins 
            alveolin 1 and 2 from Tetrahymena thermophila
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 6355)
  AUTHORS   Gould SB, McFadden GI, Waller RF.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2009) School of Botany, University of Melbourne, 
            Professors Walk, Parkville, VIC 3010, Australia
REFERENCE   4  (bases 1 to 6355)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 6355)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eukaryotic 
            Cell"; date: "2013"; volume: "12"; issue: "6"; pages: "932-940"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (24-FEB-2009) School of Botany, University of Melbourne, Professors 
            Walk, Parkville, VIC 3010, Australia"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6355
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     5'UTR           6..520
                     /label=BTU1
                     /note="BTU1"
     regulatory      527..1455
                     /label=MTT promoter
                     /note="MTT promoter"
                     /regulatory_class="promoter"
     misc_feature    1456..1494
                     /label=MCS
                     /note="MCS"
     CDS             2221..2247
                     /label=9xHis
                     /note="9xHis affinity tag"
     3'UTR           2257..3490
                     /label=BTU1
                     /note="BTU1"
     promoter        complement(3509..3527)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3548..3564)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3572..3588)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3596..3626)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3641..3662)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3950..4538)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4712..5569)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5570..5674)
                     /label=AmpR promoter
     rep_origin      complement(5700..6155)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     6297..6313
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6323..6341
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"