pTT-PB-SOKMLN-Cer vector (V002499)

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LOCUS       V002499                13592 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002499
VERSION     V002499
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 13592)
  AUTHORS   Debowski K, Warthemann R, Lentes J, Salinas-Riester G, Dressel R,
            Langenstroth D, Gromoll J, Sasaki E, Behr R.
  TITLE     Non-Viral Generation of Marmoset Monkey iPS Cells by a
            Six-Factor-in-One-Vector Approach
  JOURNAL   PLoS ONE 10 (3), E0118424 (2015)
   PUBMED   25785453
REFERENCE   2  (bases 1 to 13592)
  AUTHORS   Debowski K.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-AUG-2014) Stem Cell Biology Unit, German Primate
            Center, Kellnerweg 4, Goettingen, Niedersachsen 37077, Germany
REFERENCE   3  (bases 1 to 13592)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13592)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2015"; volume: "10"; issue: "3"; pages: "E0118424"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (11-AUG-2014) Stem Cell Biology Unit, German Primate Center,
            Kellnerweg 4, Goettingen, Niedersachsen 37077, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13592
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..318
                     /label="tight TRE promoter"
                     /note="Tet-responsive promoter PTight, consisting of seven
                     tet operator sequences followed by the minimal CMV
                     promoter"
     repeat_region   345..871
                     /label="piggyBac 5' terminal repeat"
                     /note="piggyBac 5' terminal repeat"
     enhancer        904..1283
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1286..1561
                     /label="chicken beta-actin promoter"
     intron          1562..2573
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
     CDS             2696..3652
                     /label="mSox2"
                     /note="Mus musculus transcription factor SOX-2 gene.
                     Belongs to the SRY-related HMG-box (SOX) family of
                     transcription factors, which is involved in the regulation
                     of embryonic development and in the determination of cell
                     fate"
     CDS             3653..3718
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus
                     polyprotein"
                     /label="F2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             3725..4804
                     /label="hOct4"
                     /note="Homo sapiens Oct-4 gene. Encodes a transcription
                     factor containing a POU homeodomain that plays a key role
                     in embryonic development and stem cell pluripotency.
                     Aberrant expression in adult tissues is associated with
                     tumorigenesis."
     CDS             4805..4858
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid
                     protein"
                     /label="T2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6269..6328
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus
                     polyprotein"
                     /label="E2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             6338..7651
                     /gene="MYC"
                     /label="Myc proto-oncogene protein"
                     /note="Myc proto-oncogene protein from Callithrix jacchus.
                     Accession#: P49032"
     CDS             7658..7711
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid
                     protein"
                     /label="T2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7712..8338
                     /label="hLIN28A"
                     /note="Human lin-28 homolog A gene. Encodes a LIN-28 family
                     RNA-binding protein that acts as a posttranscriptional
                     regulator of genes involved in development, self-renewal of
                     embryonic stem cells and metabolism. Overexpressed in human
                     embryonic stem cells, primary human tumors and human cancer
                     cell lines."
     CDS             8345..8398
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid
                     protein"
                     /label="T2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    9340..9925
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             9938..10654
                     /label="mCerulean"
                     /note="enhanced monomeric variant of CFP (Rizzo et al.,
                     2004)"
     polyA_signal    10689..10913
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   10939..11388
                     /label="piggyBac 3' terminal repeat"
                     /note="piggyBac 3' terminal repeat"
     polyA_signal    11506..11587
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(11769..12357)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(12531..13388)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(13389..13493)
                     /label="AmpR promoter"