pTS1 vector (V002516)

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LOCUS       V002516                 8893 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002516
VERSION     V002516
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8893)
  AUTHORS   Scott TA, Heine D, Qin Z, Wilkinson B.
  TITLE     An L-threonine transaldolase is required for
            L-threo-beta-hydroxy-alpha-amino acid assembly during obafluorin
            biosynthesis
  JOURNAL   Nat Commun 8, 15935 (2017)
   PUBMED   28649989
REFERENCE   2  (bases 1 to 8893)
  AUTHORS   Scott TA.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2016) Molecular Microbiology, John Innes Centre,
            Norwich Research Park, Norwich, Norfolk NR4 7UH, United Kingdom
REFERENCE   3  (bases 1 to 8893)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8893)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun
            8, 15935 (2017)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (30-SEP-2016) Molecular Microbiology, John Innes Centre, Norwich
            Research Park, Norwich, Norfolk NR4 7UH, United Kingdom"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8893
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(724..1371)
                     /label="TetR"
                     /note="tetracycline resistance regulatory protein"
     CDS             1477..2673
                     /label="TcR"
                     /note="tetracycline efflux protein"
     CDS             2679..2825
                     /codon_start=1
                     /gene="exc2"
                     /product="Exc2"
                     /label="exc2"
                     /protein_id="AQZ26572.1"
                     /translation="METIGNSLRGTTQFAGTDYRSKDLTPKKSRLLADTISAVYLDGYE
                     GRQ"
     gene            2679..2825
                     /gene="exc2"
                     /label="exc2"
     misc_feature    complement(2800..3083)
                     /label="cer region"
                     /note="ColE1-derived recombination site that helps to
                     maintain plasmids as monomers"
     CDS             complement(3039..4589)
                     /gene="mbeA"
                     /label="DNA relaxase MbeA"
                     /note="DNA relaxase MbeA from Escherichia coli. Accession#:
                     P13658"
     CDS             complement(4582..4926)
                     /gene="mbeC"
                     /label="Mobilization protein MbeC"
                     /note="Mobilization protein MbeC from Escherichia coli.
                     Accession#: P13657"
     CDS             4966..5154
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     oriT            5242..5330
                     /note="oriT"
     rep_origin      complement(5574..6164)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             6369..6710
                     /codon_start=1
                     /gene="colE1"
                     /product="colicin E1 immunity protein"
                     /label="colE1"
                     /protein_id="AQZ26577.1"
                     /translation="MSLRYYIKNILFGLYCTLIYIYLITKNSEGYYFLVSDKMLYAIVI
                     STILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
     gene            6369..6710
                     /gene="colE1"
                     /label="colE1"
     CDS             complement(6890..8308)
                     /label="SacB"
                     /note="secreted levansucrase that renders bacterial growth
                     sensitive to sucrose"
     promoter        complement(8309..8754)
                     /label="sacB promoter"
                     /note="sacB promoter and control region"
     misc_feature    8777..8833
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"