pTRG vector (V002527)

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LOCUS       40924_44157        4392 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTRG, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4392)
  AUTHORS   Grafsky AJ III.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-MAR-2001) Technical Services, Stratagene, 11011 N. 
            Torrey Pines Rd., La Jolla, CA 92037, USA
REFERENCE   2  (bases 1 to 4392)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4392)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (15-MAR-2001) Technical Services, Stratagene, 11011 N. Torrey Pines 
            Rd., La Jolla, CA 92037, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4392
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      47..76
                     /label=Ipp
                     /note="Ipp"
                     /regulatory_class="promoter"
     promoter        119..149
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    157..173
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     181..197
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    243..992
                     /label=RNAP alpha subunit
                     /note="RNAP alpha subunit"
     primer_bind     957..974
                     /label=forward
                     /note="forward"
     misc_feature    993..1058
                     /label=multiple cloning site
                     /note="multiple cloning site"
     primer_bind     complement(1102..1119)
                     /label=reverse
                     /note="reverse"
     rep_origin      1274..1862
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(2048..2188)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(2293..2481)
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     CDS             complement(3123..4310)
                     /label=TcR
                     /note="tetracycline efflux protein"
     promoter        complement(4358..4386)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"