pTREXL-Hyg vector (V002530)

LOCUS       40924_44142        6749 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTREXL-Hyg, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6749)
  AUTHORS   Bouvier LA, de los Milagros Camara M, Canepa GE, Miranda MR, Pereira
            CA.
  TITLE     Plasmids and molecular building blocks to assist the development and
            extension of genetic manipulation tools for Trypanosoma cruzi
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6749)
  AUTHORS   Bouvier LA, de los Milagros Camara M, Canepa GE, Miranda MR, Pereira
            CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2011) Laboratorio de Biologia Molecular de 
            Trypanosoma cruzi, Instituto de Investigaciones Medicas (IDIM) 
            Alfredo Lanari, Combatientes de Malvinas 3150, CABA, Buenos Aires 
            1427, Argentina
REFERENCE   3  (bases 1 to 6749)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6749)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-AUG-2011) Laboratorio de Biologia Molecular de Trypanosoma 
            cruzi, Instituto de Investigaciones Medicas (IDIM) Alfredo Lanari, 
            Combatientes de Malvinas 3150, CABA, Buenos Aires 1427, Argentina"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6749
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      2..811
                     /gene="ribosomal promoter"
                     /regulatory_class="promoter"
     gene            2..811
                     /gene="ribosomal promoter"
                     /label=ribosomal promoter
     intron          862..1033
                     /gene="HX1"
     gene            862..1033
                     /gene="HX1"
                     /label=HX1
     CDS             1063..1779
                     /label=EGFP
                     /note="enhanced GFP"
     intron          1819..2331
                     /gene="IgapdhI-II"
     gene            1819..2331
                     /gene="IgapdhI-II"
                     /label=IgapdhI-II
     CDS             2332..3354
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     intron          3358..3875
                     /gene="IgapdhII"
     gene            3358..3875
                     /gene="IgapdhII"
                     /label=IgapdhII
     promoter        complement(3912..3930)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3951..3967)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3975..3991)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3999..4029)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4044..4065)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4353..4941)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5115..5972)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5973..6077)
                     /label=AmpR promoter
     rep_origin      complement(6103..6558)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     6700..6716
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6723..6741
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"