Basic Vector Information
- Vector Name:
- pTRE-TALER21-4xTarget_FF3
- Antibiotic Resistance:
- Ampicillin
- Length:
- 10140 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Li Y, Jiang Y, Chen H, Liao W, Li Z, Weiss R, Xie Z.
- Promoter:
- T3
pTRE-TALER21-4xTarget_FF3 vector Map
pTRE-TALER21-4xTarget_FF3 vector Sequence
LOCUS 40924_44038 10140 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pTRE-TALER21-4xTarget_FF3, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10140)
AUTHORS Li Y, Jiang Y, Chen H, Liao W, Li Z, Weiss R, Xie Z.
TITLE Modular construction of mammalian gene circuits using TALE
transcriptional repressors
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 10140)
AUTHORS Li Y, Jiang Y, Chen H, Liao W.
TITLE Direct Submission
JOURNAL Submitted (03-SEP-2014) Bioinformatics Division/Center for Synthetic
REFERENCE 3 (bases 1 to 10140)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 10140)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(03-SEP-2014) Bioinformatics Division/Center for Synthetic "
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..10140
/mol_type="other DNA"
/organism="synthetic DNA construct"
protein_bind 1..21
/label=attB4
/note="core recombination site for the Gateway(R) BP
reaction"
protein_bind 66..84
/label=tet operator
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 101..119
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 137..155
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 173..191
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 208..226
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 244..262
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
promoter 274..312
/label=minimal CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
protein_bind 352..376
/label=attB1
/note="recombination site for the Gateway(R) BP reaction"
misc_feature 429..3764
/label=similar to TALER21
/note="similar to TALER21"
CDS 3774..3794
/codon_start=1
/label=SV40 NLS
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/translation="PKKKRKV"
misc_feature 3840..3860
/label=FF3
/note="FF3"
misc_feature 3866..3886
/label=FF3
/note="FF3"
misc_feature 3892..3912
/label=FF3
/note="FF3"
misc_feature 3918..3938
/label=FF3
/note="FF3"
protein_bind complement(3974..3998)
/label=attB2
/note="recombination site for the Gateway(R) BP reaction"
polyA_signal 4153..4208
/label=beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
primer_bind complement(4569..4585)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 4593..4609
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4617..4647)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4662..4683
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
misc_feature 4856..5692
/label=HA-R
/note="right homology arm from the adeno-associated virus
integration site (AAVS1) within intron 1 of the human
PPP1R12C gene"
promoter complement(5722..5740)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(5761..5777)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 5785..5801
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5809..5839)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(5854..5875)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(6163..6751)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(6925..7782)
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(7783..7887)
/label=AmpR promoter
rep_origin complement(7913..8368)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 8510..8526
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 8536..8554
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 8569..9372
/label=HA-L
/note="left homology arm from the adeno-associated virus
integration site (AAVS1) within intron 1 of the human
PPP1R12C gene"
misc_feature 9451..9476
/label=SA
/note="splice acceptor site"
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