pTrc-mdh vector (V002551)

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LOCUS       V002551                 5092 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002551
VERSION     V002551
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5092)
  AUTHORS   Kim B, Binkley R, Kim HU, Lee SY.
  TITLE     Metabolic engineering of Escherichia coli for the enhanced
            production of l-tyrosine
  JOURNAL   Biotechnol. Bioeng. (2018) In press
   PUBMED   30019750
REFERENCE   2  (bases 1 to 5092)
  AUTHORS   Yang D, Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2018) Dept. Chemical and Biomolecular Engineering,
            Korea Advanced Institute of Science and Technology, Daehak-ro 291,
            Daejeon 34141, South Korea
REFERENCE   3  (bases 1 to 5092)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5092)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
            Bioeng. (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-JUN-2018) Dept. Chemical and Biomolecular Engineering, Korea
            Advanced Institute of Science and Technology, Daehak-ro 291, Daejeon
            34141, South Korea"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5092
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        193..222
                     /label="trc promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    230..246
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             267..1202
                     /gene="mdh"
                     /label="Malate dehydrogenase"
                     /note="Malate dehydrogenase from Escherichia coli O6:H1
                     (strain CFT073 / ATCC 700928 / UPEC). Accession#: P61890"
     terminator      1445..1531
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1623..1650
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        1670..1761
                     /label="AmpR promoter"
     CDS             1762..2619
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      2793..3381
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(3567..3707)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     promoter        3893..3970
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             3971..5050
                     /label="lacI"
                     /note="lac repressor"