pTrc-At4CL1m-AtCHI-PhCHS vector (V002557)

LOCUS       V002557                 7827 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002557
VERSION     V002557
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7827)
  AUTHORS   Kim B, Binkley R, Kim HU, Lee SY.
  TITLE     Metabolic engineering of Escherichia coli for the enhanced
            production of l-tyrosine
  JOURNAL   Biotechnol. Bioeng. (2018) In press
   PUBMED   30019750
REFERENCE   2  (bases 1 to 7827)
  AUTHORS   Yang D, Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2018) Dept. Chemical and Biomolecular Engineering,
            Korea Advanced Institute of Science and Technology, Daehak-ro 291,
            Daejeon 34141, South Korea
REFERENCE   3  (bases 1 to 7827)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7827)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
            Bioeng. (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-JUN-2018) Dept. Chemical and Biomolecular Engineering, Korea
            Advanced Institute of Science and Technology, Daehak-ro 291, Daejeon
            34141, South Korea"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7827
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        193..222
                     /label="trc promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    230..246
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             267..1949
                     /gene="4CL1"
                     /label="4-coumarate--CoA ligase 1"
                     /note="4-coumarate--CoA ligase 1 from Arabidopsis thaliana.
                     Accession#: Q42524"
     CDS             1978..2715
                     /gene="CHI1"
                     /label="Chalcone--flavanone isomerase 1"
                     /note="Chalcone--flavanone isomerase 1 from Arabidopsis
                     thaliana. Accession#: P41088"
     CDS             2778..3944
                     /gene="CHSA"
                     /label="Chalcone synthase A"
                     /note="Chalcone synthase A from Petunia hybrida.
                     Accession#: P08894"
     terminator      4180..4266
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      4358..4385
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        4405..4496
                     /label="AmpR promoter"
     CDS             4497..5354
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5528..6116
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(6302..6442)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     promoter        6628..6705
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             6706..7785
                     /label="lacI"
                     /note="lac repressor"