pTPTK1 vector (V002563)

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LOCUS       40924_43828        5489 bp DNA     circular SYN 18-DEC-2018
DEFINITION  E. coli-T. kodakarensis shuttle vector pTPTK1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5489)
  AUTHORS   Catchpole R, Gorlas A, Oberto J, Forterre P.
  TITLE     A series of new E. coli-Thermococcus shuttle vectors compatible with
            previously existing vectors
  JOURNAL   Extremophiles (2018) In press
  PUBMED    29497842
REFERENCE   2  (bases 1 to 5489)
  AUTHORS   Catchpole R, Gorlas A, Oberto J, Forterre P.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2018) Microbiology, Institut Pasteur, 25-28 Rue du
            Dr Roux, Paris 75015, France
REFERENCE   3  (bases 1 to 5489)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5489)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Extremophiles (2018) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-FEB-2018) Microbiology, Institut Pasteur, 25-28 Rue du Dr Roux, 
            Paris 75015, France"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5489
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(268..813)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(1130..2356)
                     /codon_start=1
                     /product="HMG-CoA reductase"
                     /label=HMG-CoA reductase
                     /protein_id="AVP12432.1"
                     /translation="MEIEEIIEKVARGEIKFHQVENYVNGDKRLATEIRRRALEKKLGI
                     QLKHIGHYSIDPNEVIGRNIENMIGVVQIPMGIAGPLKINGEYAKGEFYIPLATTEGAL
                     VASVNRGCSALTEAGGVYTTLIDDKMTRAPLLKCPNARRAREVAEWVKNNLDYLQEKAV
                     SKVTRHGKLRGVKPFIVGRNLYLRFEFETGDAMGMNMVTIASEEIMKVIEEEFPDVKYL
                     ALSGNLCVDKKPNALNFILGRGKTIIAEAVVPREIVKKKLKTTPELIAEVNYLKNLVGS
                     AQAGSYGFNAHFANIVGAIFLATGQDEAQITEGAHGITLAEVTEDGDLYISITMPSLEI
                     GTVGGGTRVPPQREALEIMGVAGGGDPPGMNAKKFAEIVAGAVLAGELSLLAAIAAKHL
                     ARAHKMLGR"
     CDS             complement(2616..2750)
                     /codon_start=1
                     /product="transmembrane domain protein"
                     /label=transmembrane domain protein
                     /protein_id="AVP12431.1"
                     /translation="MSKKLDVKVKVNIYVNVHIDIKVAISIAVTIAVIALLKVLLNIR"
     CDS             2853..3002
                     /codon_start=1
                     /product="SpoVT/AbrB-like transcriptional regulator"
                     /label=SpoVT/AbrB-like transcriptional regulator
                     /note="swapped-hairpin fold CDS"
                     /protein_id="AVP12430.1"
                     /translation="MQDVRKMFRSGSSYVVAFPPSVIQKTKFRHQRTVRVRIYDDKIVI
                     VPLW"
     CDS             3115..3738
                     /codon_start=1
                     /product="putative transcriptional regulator"
                     /label=putative transcriptional regulator
                     /note="wHTH fold CDS"
                     /protein_id="AVP12429.1"
                     /translation="MCVDHRSEKLDGLKLDGRLRHVLEVVVELAGEYGGSVNLPFLARV
                     VSASTGSGSKRKTEYAKLRRDINKLRDMGLVSVEKLDGFLWVSPTPKALYLIEHRRNSN
                     RVRRSMRPEKARAIQLLRCIDIPGYFVWSKDGKNKVAEGVVNGLKGYIDYAESIRVVLV
                     RRELVVGGASTLDELGRLAVDDSDVLVVPYPYAVHVTGDACPEA"
     CDS             3665..4423
                     /codon_start=1
                     /product="RepTP2"
                     /label=RepTP2
                     /protein_id="AVP12428.1"
                     /translation="MTLMCLWFHTRTRFTSRAMLVQKLERYDSLFKVASARHTDAVFLT
                     LTTDPSRFSNLYEANRQFSHSFNRFMSRLRGYFARRGQHLEYIAVYEFTKSGLLHAHVI
                     IFGVRYLLPVRVISRWWSEAGQGRVVYIYRLRNVDGRWVWARRRPRDVRAGEGAEDYLK
                     KYLRKALRLASTLDTSDVKKVLPLALYWAFNKRFFTYSRSLLPSSRRTAEAVVSEYLWV
                     GTYRLEDLPDWVFWLPHRVYSPPSRGPPPT"
     CDS             complement(4472..5128)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(5129..5231)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"