Price Information
Cat No. | Plasmid Name | Availability | Add to cart |
---|---|---|---|
V002568 | pTOR | In stock, instant shipping |
Buy one, get one free! |
Two tubes of lyophilized plasmid will be delivered, each tube is about 5µg.
Basic Vector Information
- Vector Name:
- pTOR
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6531 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Belbahri L, Torche S, Mauch F.
- Promoter:
- lac
pTOR vector Map
Plasmid Protocol
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5. Store the plasmid at -20 ℃.
6. The concentration of plasmid re-measurement sometimes differs from the nominal value, which may be due to the position of the lyophilized plasmid in the tube, the efficiency of the re-dissolution, the measurement bias, and adsorption on the wall of the tube, therefore, it is recommended to transform and extract the plasmid before using it
General Plasmid Transform Protocol
1. Take one 100μl of the competent cells and thaw it on ice for 10min, add 2μl of plasmid, then ice bath for 30min, then heat-shock it at 42℃ for 60s, do not stir, and then ice bath for 2min.
2. Add 900μl of LB liquid medium without antibiotics, and incubate at 37℃ for 45min (30℃ for 1-1.5 hours) with 180rpm shaking.
3. Centrifuge at 6000rpm for 5min, leave only 100μl of supernatant to resuspend the bacterial precipitate and spread it onto the target plasmid-resistant LB plate.
4. Invert the plate and incubate at 37℃ for 14h, or at 30℃ for 20h.
5. Pick a single colony into LB liquid medium, add the corresponding antibiotics, incubate at 220rpm for 14h, and extract the plasmid according to the experimental needs and the instructions of the plasmid extraction kit.
pTOR vector Sequence
LOCUS Exported 6531 bp DNA circular SYN 31-AUG-2024 DEFINITION Cloning vector pTOR, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6531) AUTHORS Belbahri L, Torche S, Mauch F. TITLE Versatile cloning vectors for Phytophthora transformation JOURNAL Unpublished REFERENCE 2 (bases 1 to 6531) AUTHORS Belbahri L, Torche S, Mauch F. TITLE Direct Submission JOURNAL Submitted (31-OCT-2007) Laboratory of Applied Genetics, School of Engineering of Lullier, 150 Route de Presinge, Jussy 1254, Switzerland REFERENCE 3 (bases 1 to 6531) TITLE Direct Submission REFERENCE 4 (bases 1 to 6531) TITLE Direct Submission REFERENCE 5 (bases 1 to 6531) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (31-OCT-2007) Laboratory of Applied Genetics, School of Engineering of Lullier, 150 Route de Presinge, Jussy 1254, Switzerland" COMMENT SGRef: number: 3; type: "Journal Article" COMMENT SGRef: number: 4; type: "Journal Article" FEATURES Location/Qualifiers source 1..6531 /mol_type="other DNA" /organism="synthetic DNA construct" rep_origin 4..459 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind 600..616 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 626..644 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 1011..1575 /label=5' Bremia lactucae Ham34 promoter /note="5' Bremia lactucae Ham34 promoter" /regulatory_class="promoter" primer_bind 1588..1604 /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1638..1654) /label=SK primer /note="common sequencing primer, one of multiple similar variants" regulatory 1689..2209 /label=3' Bremia lactucae Ham34 terminator /note="3' Bremia lactucae Ham34 terminator" /regulatory_class="terminator" regulatory 2217..2800 /label=5' Bremia lactucae Hsp70 promoter /note="5' Bremia lactucae Hsp70 promoter" /regulatory_class="promoter" CDS 2816..3607 /label=NeoR/KanR /note="aminoglycoside phosphotransferase" regulatory 3797..4016 /label=3' Bremia lactucae Hsp70 terminator /note="3' Bremia lactucae Hsp70 terminator" /regulatory_class="terminator" promoter complement(4343..4361) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(4382..4398) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(4406..4422) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4430..4460) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(4475..4496) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(4784..5372) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(5546..6403) /label=AmpR /note="beta-lactamase" promoter complement(6404..6508) /label=AmpR promoter