pTOPO-1705 vector (V002569)

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LOCUS       V002569                 9002 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002569
VERSION     V002569
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9002)
  AUTHORS   Tian L, Lo J, Shao X, Zheng T, Olson DG, Lynd LR.
  TITLE     Identification of a ferredoxin:NAD+ oxidoreductase enzyme in
            Thermoanaerobacterium saccharolyticum and its role in ethanol
            formation
  JOURNAL   Appl. Environ. Microbiol. (2016) In press
   PUBMED   27694237
REFERENCE   2  (bases 1 to 9002)
  AUTHORS   Tian L.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2016) Thayer School of Engineering, Dartmouth
            College, 14 Engineering Dr, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 9002)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9002)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol. (2016) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (22-MAY-2016) Thayer School of Engineering, Dartmouth College, 14
            Engineering Dr, Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9002
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     1..1500
     CDS             1513..1530
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             2817..3551
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     misc_recomb     3609..5046
     promoter        complement(5081..5099)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5106..5122)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             5260..5559
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             5911..6702
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     CDS             complement(6958..7815)
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7939..8527
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    8815..8836
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8851..8881
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    8889..8905
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8913..8929
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        8950..8968
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"