pTNTrpE vector (V002573)

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LOCUS       V002573                 9026 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002573
VERSION     V002573
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9026)
  AUTHORS   Catchpole R, Gorlas A, Oberto J, Forterre P.
  TITLE     A series of new E. coli-Thermococcus shuttle vectors compatible with
            previously existing vectors
  JOURNAL   Extremophiles (2018) In press
   PUBMED   29497842
REFERENCE   2  (bases 1 to 9026)
  AUTHORS   Catchpole R, Gorlas A, Oberto J, Forterre P.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2018) Microbiology, Institut Pasteur, 25-28 Rue du
            Dr Roux, Paris 75015, France
REFERENCE   3  (bases 1 to 9026)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9026)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Extremophiles (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (05-FEB-2018) Microbiology, Institut Pasteur, 25-28 Rue du Dr Roux,
            Paris 75015, France"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9026
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      17..605
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    893..914
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        929..959
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    967..983
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     991..1007
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             1586..2236
                     /codon_start=1
                     /product="p24"
                     /label="p24"
                     /protein_id="AVP12426.1"
                     /translation="MESGENTPYKKFICLHCGHVFDSDRDAPKCPNCGRRKVIPRETLG
                     ESLRKHKDKLKELGVLPEPDPEPQETEVGKAEVETAGQKDPETSKPEPIQVEQKVEELK
                     PESDQEPQNSESVDSPDDVPESDKLLAKYLEELEDVPAKPKPKKLRPKKSKRSITVPAG
                     PSFLEVLIIIGAIVLIWKWLSSRSSKSDDESNVPRPSETYYQIQRNLGHHVLG"
     CDS             2771..4696
                     /codon_start=1
                     /product="Rep74"
                     /label="Rep74"
                     /protein_id="AVP12425.1"
                     /translation="MRLSNSSINSLLSSGSCDVVGSSPSGSPFLPQSTGSTQKTLSVYL
                     DISPTNEKGEPSVRGPTIRVSSHPLPAYAYYRSATGHLERQKFESQGRVKIDIYKLRKK
                     LFWIRRRLERVDLTDEERSSLEAERDRLVSLTEKLLRKIYSGSALYGDRFVIDVPVAYS
                     QLCKALGINPSDVGVSLFVRSAVMDLEFDDSSRSALKISYVSRLFAGKYHPAKGISKGF
                     KEARRVVRDLLVLQEFLDGSLLSYHKGDYVTSNSLLPMRFFVLTLPEEISYYIWSKLRE
                     GDDSALKLFKKISSQAIRDFLFYLAQKEGIPINKSYFVPGFLQNIHPTGDRDPFKPHFH
                     AHFSVVFVVYDKSSHTWYRLNPVLDEADLEKLREIWKALVVEAFSEILSGDTLTKDFNV
                     WVGDRYYSLPHDYVGVLFEIKYNARKMFVNYSSYYERNSFSDDFDRSFVSFIFDYKNRT
                     ERYGFLTNIKRYLSRLSISAVKKRLEELRELLDRIEADLLVVDSGRFPLLYQSLLDKRE
                     AVQSEISRLESVLNNPDDAFRVLYDEISRDVESMLSPRTVKENRIVSLLESLHGKRVVG
                     LSVEYIRYLTRDGEEVLDVPLHKFLEARRSVVLLSDRHKTVEFMLWWDPFWEDPPDVLE
                     LKIPNS"
     CDS             4859..6157
                     /gene="trpE"
                     /label="Anthranilate synthase component 1"
                     /note="Anthranilate synthase component 1 from Thermococcus
                     kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1).
                     Accession#: Q9YGB3"
     promoter        complement(6246..6264)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6271..6287)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      6428..6856
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             7200..7991
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     CDS             8012..8869
                     /label="AmpR"
                     /note="beta-lactamase"