pTNS2-ColE1 vector (V002577)

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LOCUS       V002577                10445 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002577
VERSION     V002577
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10445)
  AUTHORS   Lee KW, Periasamy S, Mukherjee M, Xie C, Kjelleberg S, Rice SA.
  TITLE     Biofilm development and enhanced stress resistance of a model,
            mixed-species community biofilm
  JOURNAL   ISME J 8 (4), 894-907 (2014)
   PUBMED   24152718
REFERENCE   2  (bases 1 to 10445)
  AUTHORS   Lee KWK., Periasamy S, Mukherjee M, Xie C, Kjelleberg S, Rice SA.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-SEP-2014) Singapore Centre on Environmental Life
            Sciences Engineering, Nanyang Technological University, 60 Nanyang
            Drive, SBS-01N-27 637551, Singapore
REFERENCE   3  (bases 1 to 10445)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10445)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ISME J";
            date: "2014"; volume: "8"; issue: "4"; pages: "894-907"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-SEP-2014) Singapore Centre on Environmental Life Sciences
            Engineering, Nanyang Technological University, 60 Nanyang Drive,
            SBS-01N-27 637551, Singapore"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10445
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(690..2216)
                     /codon_start=1
                     /gene="tnsD"
                     /product="TnsD"
                     /label="tnsD"
                     /note="Tn7 transposition site selector"
                     /protein_id="AIZ73053.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVAT
                     LGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQGAV
                     HLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVFFDR
                     AVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQWTLFY
                     QRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFRKHRKA
                     FSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVKHKDWQQ
                     LVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDWNQRDRIA
                     VRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKRYSESVEDY
                     QIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE"
     gene            complement(690..2216)
                     /gene="tnsD"
                     /label="tnsD"
     CDS             complement(2222..3886)
                     /gene="tnsC"
                     /label="Transposon Tn7 transposition protein TnsC"
                     /note="Transposon Tn7 transposition protein TnsC from
                     Escherichia coli. Accession#: P05846"
     CDS             complement(3886..5991)
                     /gene="tnsB"
                     /label="Transposon Tn7 transposition protein TnsB"
                     /note="Transposon Tn7 transposition protein TnsB from
                     Escherichia coli. Accession#: P13989"
     mobile_element  6709..6933
                     /label="Tn7R"
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     rep_origin      complement(7197..7785)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     primer_bind     complement(7971..7987)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    7995..8011
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8019..8049)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8064..8085)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     oriT            8461..8569
                     /label="oriT"
                     /note="incP origin of transfer"
     rep_origin      8592..8976
                     /label="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     CDS             complement(9388..10245)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(10246..10350)
                     /label="AmpR promoter"