pTNAg vector (V002578)

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LOCUS       40924_43738        8189 bp DNA     circular SYN 18-DEC-2018
DEFINITION  E. coli-T. kodakarensis shuttle vector pTNAg, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8189)
  AUTHORS   Catchpole R, Gorlas A, Oberto J, Forterre P.
  TITLE     A series of new E. coli-Thermococcus shuttle vectors compatible with
            previously existing vectors
  JOURNAL   Extremophiles (2018) In press
  PUBMED    29497842
REFERENCE   2  (bases 1 to 8189)
  AUTHORS   Catchpole R, Gorlas A, Oberto J, Forterre P.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2018) Microbiology, Institut Pasteur, 25-28 Rue du
            Dr Roux, Paris 75015, France
REFERENCE   3  (bases 1 to 8189)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8189)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Extremophiles (2018) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-FEB-2018) Microbiology, Institut Pasteur, 25-28 Rue du Dr Roux, 
            Paris 75015, France"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8189
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      17..605
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    893..914
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        929..959
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    967..983
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     991..1007
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1586..2236
                     /codon_start=1
                     /product="p24"
                     /label=p24
                     /protein_id="AVP12421.1"
                     /translation="MESGENTPYKKFICLHCGHVFDSDRDAPKCPNCGRRKVIPRETLG
                     ESLRKHKDKLKELGVLPEPDPEPQETEVGKAEVETAGQKDPETSKPEPIQVEQKVEELK
                     PESDQEPQNSESVDSPDDVPESDKLLAKYLEELEDVPAKPKPKKLRPKKSKRSITVPAG
                     PSFLEVLIIIGAIVLIWKWLSSRSSKSDDESNVPRPSETYYQIQRNLGHHVLG"
     CDS             2771..4696
                     /codon_start=1
                     /product="Rep74"
                     /label=Rep74
                     /protein_id="AVP12420.1"
                     /translation="MRLSNSSINSLLSSGSCDVVGSSPSGSPFLPQSTGSTQKTLSVYL
                     DISPTNEKGEPSVRGPTIRVSSHPLPAYAYYRSATGHLERQKFESQGRVKIDIYKLRKK
                     LFWIRRRLERVDLTDEERSSLEAERDRLVSLTEKLLRKIYSGSALYGDRFVIDVPVAYS
                     QLCKALGINPSDVGVSLFVRSAVMDLEFDDSSRSALKISYVSRLFAGKYHPAKGISKGF
                     KEARRVVRDLLVLQEFLDGSLLSYHKGDYVTSNSLLPMRFFVLTLPEEISYYIWSKLRE
                     GDDSALKLFKKISSQAIRDFLFYLAQKEGIPINKSYFVPGFLQNIHPTGDRDPFKPHFH
                     AHFSVVFVVYDKSSHTWYRLNPVLDEADLEKLREIWKALVVEAFSEILSGDTLTKDFNV
                     WVGDRYYSLPHDYVGVLFEIKYNARKMFVNYSSYYERNSFSDDFDRSFVSFIFDYKNRT
                     ERYGFLTNIKRYLSRLSISAVKKRLEELRELLDRIEADLLVVDSGRFPLLYQSLLDKRE
                     AVQSEISRLESVLNNPDDAFRVLYDEISRDVESMLSPRTVKENRIVSLLESLHGKRVVG
                     LSVEYIRYLTRDGEEVLDVPLHKFLEARRSVVLLSDRHKTVEFMLWWDPFWEDPPDVLE
                     LKIPNS"
     CDS             4923..5396
                     /codon_start=1
                     /product="pyruvoyl-dependent arginine decarboxylase"
                     /label=pyruvoyl-dependent arginine decarboxylase
                     /protein_id="AVP12419.1"
                     /translation="MSWTTPKRAFIGAATAEGGTKLNAFDNALLKLGIGNVNLVKLSSV
                     IPAHIEWMEKVHDVPIGMLLPTVYAHIESDEPGMTISAALGVGISKNNEGGLIYEYSGY
                     CTKEEAEEMVRKMVEEGFRQRGWELGEFKVASAEITVKDKPAAAIAVVVMFPY"
     promoter        complement(5409..5427)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5434..5450)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      5591..6019
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             6363..7154
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7175..8032
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"