Basic Vector Information
- Vector Name:
- enIscB
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7501 bp
- Type:
- Protein expression, RNA expression
- Replication origin:
- ori
- Host:
- Mammalian cells
- Promoter:
- CAG, hU6, CMV
- 5' Primer:
- CMV-F
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
enIscB vector Map
enIscB vector Sequence
LOCUS . 7501 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
enhancer 227..512
/label="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer;
contains an 18-bp deletion relative to the standard CMV
enhancer"
promoter 514..791
/label="chicken β-actin promoter"
CDS 1040..1105
/label="3xFLAG"
/note="three tandem FLAG® epitope tags, followed by an
enterokinase cleavage site"
CDS 1109..1129
/label="SV40 NLS"
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
CDS 2618..2665
/label="nucleoplasmin NLS"
/note="bipartite nuclear localization signal from
nucleoplasmin"
polyA_signal 2699..2906
/label="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
promoter 2907..3147
/label="U6 promoter"
/note="RNA polymerase III promoter for human U6 snRNA"
enhancer 3446..3749
/label="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 3750..3953
/label="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 3982..4689
/label="mCherry"
/note="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
polyA_signal 4702..5155
/label="PGK poly(A) signal"
/note="mouse phosphoglycerate kinase 1 polyadenylation
signal"
rep_origin 5247..5702
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5984..6088
/label="AmpR promoter"
CDS 6089..6946
/label="AmpR"
/note="β-lactamase"
rep_origin 7120..7708
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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