PiggyBac-CAG-GFP11x7-Puro vector (V018079)

LOCUS       .                       8037 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        complement(1..105)
                     /label="AmpR promoter"
     rep_origin      complement(131..586)
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     728..744
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     intron          2100..3111
                     /label="chimeric intron"
                     /note="chimera between introns from chicken β-actin and
                     rabbit β-globin"
     CDS             3368..3793
                     /label="GFP11x7"
                     /note="seven tandem copies of the 11th β-strand of
                     superfolder GFP"
     CDS             3847..3900
                     /label="T2A"
                     /note="2A peptide from Thosea asigna virus capsid protein"
     CDS             3901..4497
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     polyA_signal    4608..4740
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6016..6032)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6040..6056)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6064..6094)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6109..6130)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6418..7006)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7180..8037)
                     /label="AmpR"
                     /note="β-lactamase"