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37825-GFP vector (V018029)

Basic Vector Information

Vector Name:
37825-GFP
Antibiotic Resistance:
Ampicillin
Length:
5207 bp
Type:
Protein expression
Replication origin:
ori
Host:
Mammalian cells, Adeno-associated virus
Promoter:
CAG
3' Primer:
WPRE-R
Growth Strain(s):
Stbl3
Growth Temperature:
37℃

37825-GFP vector Map

Copy Sequence View Map
37825-GFP5207 bp6001200180024003000360042004800oriAmpRAmpR promoterf1 orichicken β-actin promoterEGFPWPRESV40 poly(A) signal

37825-GFP vector Sequence

LOCUS       .                       5207 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     rep_origin      complement(182..770)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(944..1801)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(1802..1906)
                     /label="AmpR promoter"
     rep_origin      complement(1932..2387)
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2899..3176
                     /label="chicken β-actin promoter"
     CDS             3383..4099
                     /label="EGFP"
                     /note="the original enhanced GFP (Yang et al., 1996)"
     misc_feature    4109..4697
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     polyA_signal    complement(4740..4861)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"

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