Basic Vector Information
- Vector Name:
- pX330-BbsI-PITCh
- Antibiotic Resistance:
- Ampicillin
- Length:
- 8947 bp
- Type:
- Gene-editing vector
- Replication origin:
- ori
- Host:
- Mammalian cells
- Promoter:
- U6
- 3' Primer:
- enCMV-R
- Growth Strain(s):
- Stbl3
- Growth Temperature:
- 37℃
pX330-BbsI-PITCh vector Map
pX330-BbsI-PITCh vector Sequence
LOCUS . 8947 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
intron 208..435
/label="hybrid intron"
/note="hybrid between chicken β-actin (CBA) and minute
virus of mice (MMV) introns (Gray et al., 2011)"
CDS 573..4673
/label="Cas9"
/note="S. pyogenes Cas9, codon optimized for C. elegans
with synthetic introns"
polyA_signal 4755..4962
/label="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
repeat_region 4971..5111
/label="AAV2 ITR"
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 5186..5641
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5923..6027
/label="AmpR promoter"
CDS 6028..6885
/label="AmpR"
/note="β-lactamase"
rep_origin 7059..7647
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_RNA 7976..8051
/label="gRNA scaffold"
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
promoter 8149..8389
/label="U6 promoter"
/note="RNA polymerase III promoter for human U6 snRNA"
enhancer 8590..8875
/label="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer;
contains an 18-bp deletion relative to the standard CMV
enhancer"
promoter 8877..9154
/label="chicken β-actin promoter"
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