Basic Vector Information
- Vector Name:
- pX333-2×(U6-gRNA)-CBH-Cas9-Puro-EGFP
- Antibiotic Resistance:
- Ampicillin
- Length:
- 10439 bp
- Type:
- Gene-editing vector
- Host:
- Mammalian cells
- Selection Marker:
- Puro
- Promoter:
- U6
- 3' Primer:
- BGH-R
- Growth Strain(s):
- Stbl3
- Growth Temperature:
- 37℃
pX333-2×(U6-gRNA)-CBH-Cas9-Puro-EGFP vector Map
pX333-2×(U6-gRNA)-CBH-Cas9-Puro-EGFP vector Sequence
LOCUS . 10439 bp DNA linear UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
misc_RNA 337..412
/label="gRNA scaffold"
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
promoter 503..743
/label="U6 promoter"
/note="RNA polymerase III promoter for human U6 snRNA"
enhancer 943..1228
/label="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer;
contains an 18-bp deletion relative to the standard CMV
enhancer"
promoter 1230..1507
/label="chicken β-actin promoter"
CDS 1873..5973
/label="Cas9"
/note="S. pyogenes Cas9, codon optimized for C. elegans
with synthetic introns"
CDS 6754..7470
/label="EGFP"
/note="the original enhanced GFP (Yang et al., 1996)"
polyA_signal 7504..7711
/label="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
repeat_region 7720..7860
/label="AAV2 ITR"
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 7935..8390
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 8672..8776
/label="AmpR promoter"
CDS 8777..9634
/label="AmpR"
/note="β-lactamase"
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