Basic Vector Information
The plasmid pYES2-EGFP is a high-copy, episomal yeast expression vector. It utilizes the galactose-inducible GAL1 promoter to control the expression of Enhanced Green Fluorescent Protein (EGFP) in Saccharomyces cerevisiae. It carries both URA3 marker and Nourseothricin (NrsR) for yeast selection.
- Vector Name:
- pYES2-EGFP-Nat
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7697 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Yeast
- Selection Marker:
- URA3 and NrsR
- Promoter:
- GAL1
- 5' Primer:
- T7
- Growth Strain(s):
- DH10B
- Growth Temperature:
- 37℃
pYES2-EGFP-Nat vector Map
pYES2-EGFP-Nat vector Sequence
LOCUS . 7697 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
rep_origin complement(237..825)
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature complement(1294..1672)
/label="ISS"
/note="immunostimulatory sequence from the AmpR gene;
contains unmethylated CpG dinucleotides in the context of
5'-AACGTT-3' (Sato et al., 1996)"
CDS complement(1955..2755)
/label="URA3"
/note="orotidine-5'-phosphate decarboxylase, required for
uracil biosynthesis"
promoter complement(2756..2976)
/label="URA3 promoter"
promoter 2985..3328
/label="TEF promoter"
/note="Ashbya gossypii TEF promoter"
CDS 3348..3911
/label="NrsR"
/note="nourseothricin acetyltransferase"
terminator 3929..4126
/label="TEF terminator"
/note="Ashbya gossypii TEF terminator"
rep_origin complement(4723..5603)
/label="2μ ori"
/note="yeast 2μ plasmid origin of replication"
rep_origin complement(5614..6127)
/label="M13 ori"
/note="M13 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 6146..6588
/label="GAL1 promoter"
/note="inducible promoter, regulated by Gal4"
promoter 6620..6638
/label="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 6670..7383
/label="GFP (S65T)"
/note="S65T variant of Aequorea victoria green fluorescent
protein (Heim et al., 1995)"
terminator 7446..7693
/label="CYC1 terminator"
/note="transcription terminator for CYC1"
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