pYLsGRNA-ATU3b/LACZ vector (V017882)

LOCUS       .                       3369 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_RNA        20..95
                     /label="gRNA scaffold"
                     /note="guide RNA scaffold for the Streptococcus pyogenes
                     CRISPR/Cas9 system"
     primer_bind     complement(723..739)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        1213..1317
                     /label="AmpR promoter"
     CDS             1318..2175
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      2349..2937
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    3225..3246
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3261..3291
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    3299..3315
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     primer_bind     3323..3339
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"