TERT vector (V017862)

LOCUS       .                       9728 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        1..1179
                     /label="EF-1α promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1α"
     promoter        1196..1214
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1293..1317
                     /label="attB1"
                     /note="recombination site for the Gateway® BP reaction"
     CDS             complement(4336..4347)
                     /label="WELQut site"
                     /note="WELQut protease recognition and cleavage site"
     protein_bind    complement(4685..4709)
                     /label="attB2"
                     /note="recombination site for the Gateway® BP reaction"
     CDS             4834..4875
                     /label="V5 tag"
                     /note="epitope tag from simian virus 5"
     CDS             4888..5274
                     /label="MS2"
                     /note="bacteriophage MS2 coat protein"
     polyA_signal    5336..5560
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    5844..5891
                     /label="FRT"
                     /note="FLP-mediated recombination occurs in the 8-bp core
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5899..6918
                     /label="HygR"
                     /note="hygromycin B phosphotransferase"
     polyA_signal    7051..7184
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7221..7237)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(7245..7261)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7269..7299)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7314..7335)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7623..8211)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8385..9242)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(9243..9347)
                     /label="AmpR promoter"
     promoter        9730..10907
                     /label="EF-1α promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1α"