Basic Vector Information
- Vector Name:
- pYLsgRNA-AtU6-1
- Antibiotic Resistance:
- Ampicillin
- Length:
- 3146 bp
- Type:
- Gene-editing vector
- Replication origin:
- ori
- Host:
- Plants
- 5' Primer:
- M13R
- Growth Strain(s):
- Stbl3
- Growth Temperature:
- 37℃
pYLsgRNA-AtU6-1 vector Map
pYLsgRNA-AtU6-1 vector Sequence
LOCUS . 3146 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
misc_RNA 20..95
/label="gRNA scaffold"
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
promoter 162..465
/label="AtU6-1 promoter"
/note="promoter for the Arabidopsis thaliana U6-1 snRNA
gene (Waibel and Filipowicz, 1990)"
primer_bind complement(500..516)
/label="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
promoter 990..1094
/label="AmpR promoter"
CDS 1095..1952
/label="AmpR"
/note="β-lactamase"
rep_origin 2126..2714
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 3002..3023
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 3038..3068
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 3076..3092
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG)."
primer_bind 3100..3116
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
This page is informational only.