Basic Vector Information
- Vector Name:
- pcDNA3-N-Flag-NLRP3 711-1034
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6408 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells
- Selection Marker:
- Neo/G418
- Promoter:
- CMV
- 5' Primer:
- CMV-F
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pcDNA3-N-Flag-NLRP3 711-1034 vector Map
pcDNA3-N-Flag-NLRP3 711-1034 vector Sequence
LOCUS . 6408 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
enhancer 235..614
/label="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 863..881
/label="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 922..945
/label="FLAG"
/note="FLAG® epitope tag, followed by an enterokinase
cleavage site"
promoter complement(1960..1978)
/label="SP6 promoter"
/note="promoter for bacteriophage SP6 RNA polymerase"
polyA_signal 2004..2228
/label="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
rep_origin 2274..2702
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2716..3046
/label="SV40 promoter"
/note="SV40 enhancer and early promoter"
CDS 3113..3904
/label="NeoR/KanR"
/note="aminoglycoside phosphotransferase"
polyA_signal 4081..4214
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
primer_bind complement(4251..4267)
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(4275..4291)
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG)."
promoter complement(4299..4329)
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind complement(4344..4365)
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(4653..5241)
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5415..6272)
/label="AmpR"
/note="β-lactamase"
promoter complement(6273..6377)
/label="AmpR promoter"
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