Basic Vector Information
- Vector Name:
- MSCV-human Erbb2-IRES-GFP
- Antibiotic Resistance:
- Ampicillin
- Length:
- 10280 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells, Retrovirus
- Promoter:
- MMLV
- 5' Primer:
- pBABE-5
- Growth Strain(s):
- Stable
- Growth Temperature:
- 37℃
MSCV-human Erbb2-IRES-GFP vector Map
MSCV-human Erbb2-IRES-GFP vector Sequence
LOCUS . 10280 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
LTR 197..713
/label="5' LTR"
/note="5' long terminal repeat from murine embryonic stem
cell virus"
misc_feature 777..1118
/label="MESV Ψ"
/note="packaging signal of murine embryonic stem cell
virus"
CDS 1185..1601
/label="gag (truncated)"
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
CDS 1639..5403
/gene="ERBB2"
/label="ERBB2"
/note="Receptor tyrosine-protein kinase erbB-2 from Homo
sapiens. Accession#: P04626"
misc_feature 5426..5987
/label="IRES"
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
CDS 5990..6706
/label="EGFP"
/note="the original enhanced GFP (Yang et al., 1996)"
LTR 6871..7385
/label="3' LTR"
/note="3' long terminal repeat from murine embryonic stem
cell virus"
promoter complement(7571..7601)
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind complement(7616..7637)
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(7925..8513)
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(8687..9544)
/label="AmpR"
/note="β-lactamase"
promoter complement(9545..9649)
/label="AmpR promoter"
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