Basic Vector Information
- Vector Name:
- pMSCV-MCS-UBC-EGFP
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7121 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells, Retrovirus
- Promoter:
- MSCV
- 5' Primer:
- PLXSN-F
- Growth Strain(s):
- Stbl3
- Growth Temperature:
- 37℃
pMSCV-MCS-UBC-EGFP vector Map
pMSCV-MCS-UBC-EGFP vector Sequence
LOCUS . 7121 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
misc_feature 579..920
/label="MESV Ψ"
/note="packaging signal of murine embryonic stem cell
virus"
CDS 987..1403
/label="gag (truncated)"
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
promoter 1452..2663
/label="UbC promoter"
/note="human ubiquitin C promoter"
CDS 2717..3433
/label="EGFP"
/note="the original enhanced GFP (Yang et al., 1996)"
LTR 3483..3997
/label="3' LTR"
/note="3' long terminal repeat from murine embryonic stem
cell virus"
primer_bind complement(4166..4182)
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(4190..4206)
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG)."
promoter complement(4214..4244)
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind complement(4259..4280)
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(4568..5156)
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5330..6187)
/label="AmpR"
/note="β-lactamase"
promoter complement(6188..6292)
/label="AmpR promoter"
LTR 7120..7636
/label="5' LTR"
/note="5' long terminal repeat from murine embryonic stem
cell virus"
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