Basic Vector Information
- Vector Name:
- 139mCAR
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6992 bp
- Type:
- Protein expression, Antibody expression
- Replication origin:
- ori
- Host:
- Mammalian cells, Retrovirus
- Growth Strain(s):
- Stbl3
- Growth Temperature:
- 37℃
139mCAR vector Map
139mCAR vector Sequence
LOCUS . 6992 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
LTR 1..517
/label="5' LTR"
/note="5' long terminal repeat from murine embryonic stem
cell virus"
CDS 1020..1427
/label="gag (truncated)"
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
misc_feature 1437..1807
/label="pol region"
/note="Moloney murine leukemia virus (MMLV) pol region
containing the splice acceptor site"
CDS 1909..1932
/label="FLAG"
/note="FLAG® epitope tag, followed by an enterokinase
cleavage site"
LTR 3377..3891
/label="3' LTR"
/note="3' long terminal repeat from murine embryonic stem
cell virus"
protein_bind complement(4063..4079)
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG)."
promoter complement(4087..4117)
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind complement(4132..4153)
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(4441..5029)
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5203..6060)
/label="AmpR"
/note="β-lactamase"
promoter complement(6061..6165)
/label="AmpR promoter"
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