Basic Vector Information
- Vector Name:
- LZRS ERTm RasV12-HA
- Antibiotic Resistance:
- Ampicillin
- Length:
- 12377 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells, Retrovirus
- Selection Marker:
- Puro
- Growth Strain(s):
- Stbl3
- Growth Temperature:
- 37℃
LZRS ERTm RasV12-HA vector Map
LZRS ERTm RasV12-HA vector Sequence
LOCUS . 12377 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
CDS 445..807
/gene="ESR1"
/label="ESR1"
/note="Estrogen receptor from Macaca mulatta. Accession#:
P49886"
CDS 1045..1611
/label="H-Ras (G12V)"
/note="human oncoprotein generated by the G12V mutation in
the small GTPase H-Ras"
CDS 1612..1638
/label="HA"
/note="HA (human influenza hemagglutinin) epitope tag"
LTR 1708..2299
/label="LTR"
/note="long terminal repeat from Moloney murine leukemia
virus"
rep_origin complement(2600..3188)
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
polyA_signal complement(3311..3445)
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
CDS complement(3678..4274)
/label="PuroR"
/note="puromycin N-acetyltransferase"
promoter complement(4357..4856)
/label="PGK promoter"
/note="mouse phosphoglycerate kinase 1 promoter"
rep_origin 6888..8677
/label="oriP"
/note="Epstein-Barr virus oriP replication origin (Yates et
al., 2000)"
CDS complement(9174..10031)
/label="AmpR"
/note="β-lactamase"
promoter complement(10032..10136)
/label="AmpR promoter"
LTR 10552..11144
/label="LTR"
/note="long terminal repeat from Moloney murine leukemia
virus"
misc_feature 11207..11564
/label="MMLV Ψ"
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
CDS 11629..12045
/label="gag (truncated)"
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
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