pcDNA3 Connexin43-GFP-APEX2 vector (V017725)

LOCUS       .                       8042 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        167..546
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        547..750
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        795..813
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2022..2738
                     /label="EGFP"
                     /note="the original enhanced GFP (Yang et al., 1996)"
     CDS             2778..3524
                     /label="APEX2"
                     /note="soybean ascorbate peroxidase, engineered to serve as
                     a monomeric tag for electron microscopy and live-cell
                     proteomics (Lam et al., 2015)"
     polyA_signal    3571..3795
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3841..4269
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4283..4612
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4679..5470
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5647..5780
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5817..5833)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5841..5857)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5865..5895)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5910..5931)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6219..6807)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6981..7838)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(7839..7943)
                     /label="AmpR promoter"