pCRISPR-PE-bacteria vector (V017643)

LOCUS       .                       8838 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             join(8723..8838,1..508)
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     promoter        541..643
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             644..1300
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     terminator      1324..1418
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     rep_origin      1532..2077
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     terminator      complement(2239..2266)
                     /label="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     terminator      complement(2282..2353)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(4513..8616)
                     /label="Cas9(H840A)"
                     /note="nickase mutant of the Cas9 endonuclease from the
                     Streptococcus pyogenes Type II CRISPR/Cas system"
     promoter        complement(8652..8707)
                     /label="tetR/tetA promoters"
                     /note="overlapping promoters for bacterial tetR and tetA"