Basic Vector Information
- Vector Name:
- pBAD33-Tet
- Antibiotic Resistance:
- Chloramphenicol, Tetracycline
- Length:
- 6774 bp
- Type:
- Protein expression, Tetracycline inducible
- Replication origin:
- p15A ori
- Host:
- E. coli
- Promoter:
- BAD
- 5' Primer:
- PBAD30.F
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pBAD33-Tet vector Map
pBAD33-Tet vector Sequence
LOCUS . 6774 bp DNA circular UNK 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION VERSION KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers CDS complement(99..974) /label="araC" /note="L-arabinose regulatory protein" promoter 1001..1285 /label="araBAD promoter" /note="promoter of the L-arabinose operon of E. coli; the araC regulatory gene is transcribed in the opposite direction (Guzman et al., 1995)" misc_feature 1306..1362 /label="MCS" /note="pUC18/19 multiple cloning site" terminator 1565..1651 /label="rrnB T1 terminator" /note="transcription terminator T1 from the E. coli rrnB gene" terminator 1743..1770 /label="rrnB T2 terminator" /note="transcription terminator T2 from the E. coli rrnB gene" promoter 1789..1880 /label="AmpR promoter" promoter 2130..2234 /label="AmpR promoter" CDS 2309..3496 /label="TcR" /note="tetracycline efflux protein" rep_origin 3756..4211 /label="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" CDS complement(4769..5425) /label="CmR" /note="chloramphenicol acetyltransferase" promoter complement(5426..5528) /label="cat promoter" /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" rep_origin complement(6054..6599) /label="p15A ori" /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin."
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