pBAD33-Tet vector (V017641)

Basic Vector Information

Vector Name:
pBAD33-Tet
Antibiotic Resistance:
Chloramphenicol, Tetracycline
Length:
6774 bp
Type:
Protein expression, Tetracycline inducible
Replication origin:
p15A ori
Host:
E. coli
Promoter:
BAD
5' Primer:
PBAD30.F
Growth Strain(s):
DH5a
Growth Temperature:
37℃

pBAD33-Tet vector Map

pBAD33-Tet6774 bp3006009001200150018002100240027003000330036003900420045004800510054005700600063006600araCaraBAD promoterMCSrrnB T1 terminatorrrnB T2 terminatorAmpR promoterAmpR promoterTcRf1 oriCmRcat promoterp15A ori

pBAD33-Tet vector Sequence

LOCUS       .                       6774 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             complement(99..974)
                     /label="araC"
                     /note="L-arabinose regulatory protein"
     promoter        1001..1285
                     /label="araBAD promoter"
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite
                     direction (Guzman et al., 1995)"
     misc_feature    1306..1362
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     terminator      1565..1651
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1743..1770
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        1789..1880
                     /label="AmpR promoter"
     promoter        2130..2234
                     /label="AmpR promoter"
     CDS             2309..3496
                     /label="TcR"
                     /note="tetracycline efflux protein"
     rep_origin      3756..4211
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(4769..5425)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(5426..5528)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      complement(6054..6599)
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."

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