Basic Vector Information
- Vector Name:
- pBAD33-Tet
- Antibiotic Resistance:
- Chloramphenicol, Tetracycline
- Length:
- 6774 bp
- Type:
- Protein expression, Tetracycline inducible
- Replication origin:
- p15A ori
- Host:
- E. coli
- Promoter:
- BAD
- 5' Primer:
- PBAD30.F
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pBAD33-Tet vector Map
pBAD33-Tet vector Sequence
LOCUS . 6774 bp DNA circular UNK 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION
VERSION
KEYWORDS .
SOURCE .
ORGANISM .
.
FEATURES Location/Qualifiers
CDS complement(99..974)
/label="araC"
/note="L-arabinose regulatory protein"
promoter 1001..1285
/label="araBAD promoter"
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction (Guzman et al., 1995)"
misc_feature 1306..1362
/label="MCS"
/note="pUC18/19 multiple cloning site"
terminator 1565..1651
/label="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 1743..1770
/label="rrnB T2 terminator"
/note="transcription terminator T2 from the E. coli rrnB
gene"
promoter 1789..1880
/label="AmpR promoter"
promoter 2130..2234
/label="AmpR promoter"
CDS 2309..3496
/label="TcR"
/note="tetracycline efflux protein"
rep_origin 3756..4211
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
CDS complement(4769..5425)
/label="CmR"
/note="chloramphenicol acetyltransferase"
promoter complement(5426..5528)
/label="cat promoter"
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
rep_origin complement(6054..6599)
/label="p15A ori"
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin."
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