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hIR-GFP vector (V017630)

Basic Vector Information

Vector Name:
hIR-GFP
Antibiotic Resistance:
Kanamycin
Length:
8850 bp
Type:
Protein expression
Replication origin:
ori
Host:
Mammalian cells
Promoter:
CMV
5' Primer:
CMV-F
Growth Strain(s):
DH5a
Growth Temperature:
37℃

hIR-GFP vector Map

Copy Sequence View Map
hIR-GFP8850 bp40080012001600200024002800320036004000440048005200560060006400680072007600800084008800SV40 promoterAmpR promoterf1 oriSV40 poly(A) signalEGFPCMV promoterCMV enhanceroriHSV TK poly(A) signalNeoR/KanR

hIR-GFP vector Sequence

LOCUS       .                       8850 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        complement(join(8801..8850,1..308))
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        complement(310..414)
                     /label="AmpR promoter"
     rep_origin      441..896
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    complement(903..1024)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(1150..1866)
                     /label="EGFP"
                     /note="the original enhanced GFP (Yang et al., 1996)"
     promoter        complement(6094..6297)
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(6298..6601)
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(6776..7364)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     polyA_signal    complement(7693..7740)
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(7975..8766)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase from Tn5"

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